Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker

• 产品名称

  Anti-F4/80抗体[CI:A3-1] - Macrophage Marker
  参阅全部 F4/80 一抗

• 描述

  大鼠单克隆抗体[CI:A3-1] to F4/80 - Macrophage Marker

• 宿主

  Rat

• 特异性

  Anti-F4/80 antibody (ab6640) recognises the mouse F4/80 antigen, a 160kD glycoprotein expressed by murine macrophages. 

• 经测试应用

  适用于: Flow Cyt, ICC/IFmore details

• 种属反应性

  与反应: Mouse
  

• 免疫原

  Tissue, cells or virus corresponding to F4/80. Thioglycollate stimulated peritoneal macrophages from C57/BL mice

• 阳性对照

• ICC/IF: Raw 264.7 cells, mouse bone marrow cells. Flow Cyt: J774 cells and peritoneal macrophages.

• 常规说明

  Please note we cannot guarantee IHC-P, however should you use this application for this product then please use a specific protocol which can be found in the protocols section of our datasheet here.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.09% Sodium azide
  Constituent: PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein G purified

• 纯化说明

  Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.

• 克隆

  单克隆

• 克隆编号

  CI:A3-1

• 骨髓瘤

  NS1

• 同种型

  IgG2b

The Abpromise guarantee

Abpromise™承诺保证使用ab6640于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 13733 Mouse

• SwissProt: Q61549 Mouse

• Unigene: 2254 Mouse

• 别名

• AGRE1_HUMAN antibody

• Cell surface glycoprotein EMR1 antibody

• Cell surface glycoprotein F4/80 antibody

• DD7A5 7 antibody

• Egf like module containing mucin like hormone receptor like 1 antibody

• Egf like module containing mucin like hormone receptor like sequence 1 antibody

• EGF like module receptor 1 antibody

• EGF TM7 antibody

• EGF-like module receptor 1 antibody

• EGF-like module-containing mucin-like hormone receptor-like 1 antibody

• EGFTM7 antibody

• EMR 1 antibody

• EMR1 antibody

• EMR1 hormone receptor antibody

• Gpf480 antibody

• Ly71 antibody

• Lymphocyte antigen 71 antibody

• TM7LN3 antibody

• ADGRE1 antibody

• Adhesion G protein coupled receptor E1 antibody

• Adhesion G protein-coupled receptor E1 antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)

  ab6640 stained Raw 264.7 cells. The cells were 100% methanol fixed for 5 minutes at room temperatureand then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6640 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab150165 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed used at a 1/1000 dilution for 1 hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

• 

  Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)

  Flow cytometry analysis of J774 cells labelling F4/80 (red) with ab6640 at a dilution of 1/10 followed by streptavidin FITC secondary at 1/100 dilution. The blue line shows J774 cells stained with Rat anti mouse isotype control. 

• 

  Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)This image is courtesy of an anonymous Abreview

  ab6640 staining F4/80 in mouse bone marrow cells by immunocytochemistry/ immunofluorescence. Cells were formaldehyde fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 2% BSA for 30 minutes at 20°C. The primary antibody was diluted 1/200 and incubated with the sample for 9 hour at 4°C. Alexa fluor® 488 goat polyclonal to rat Ig, diluted 1/200, was used as the secondary antibody.

  See Abreview

• 

  Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)

  Flow cytometry analysis of peritoneal macrophages labelling F4/80 (red) with ab6640 at a dilution of 1/10. The blue line shows J774 cells stained with Rat anti mouse isotype control. 

• 

  Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)

  Flow cytometry analysis of J774 cells labelling F4/80 (red) with ab6640 at a dilution of 1/50 followed by goat anti rat IgG FITC secondary antibody at 1/100 dilution. The blue line shows J774 cells stained with Rat anti mouse isotype control.