驴F(ab)抗山羊IgG H&L (HRP)
参阅全部 IgG 二抗
Donkey
Goat
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Donkey Serum. No reaction was observed against anti-Papain or anti-Donkey IgG F(c).
适用于: WB, Dot blot, ELISA, Electron Microscopy, IHC, ICC/IFmore details
Goat IgG whole molecule
HRP
Liquid
Shipped at 4°C. Store at +4°C.
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
BSA is IgG and protease free
Affinity purified
Fab Goat IgG (H&L) Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)
多克隆
IgG
Abpromise™承诺保证使用ab6667于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | 1/2000 - 1/10000. | |
Dot blot | Use at an assay dependent dilution. | |
ELISA | 1/17000. This product has been assayed against 1.0 µg of Goat IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. | |
Electron Microscopy | Use at an assay dependent dilution. | |
IHC | Use at an assay dependent dilution. | |
ICC/IF | 1/500 - 1/2500. |