生物素Anti-Fluorescein抗体
参阅全部 Fluorescein 一抗
生物素山羊多克隆抗体to Fluorescein
Goat
Biotin
适用于: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, In situ hybridization, WBmore details
与反应: Species independent
Chemical/ Small Molecule corresponding to Fluorescein conjugated to bovine serum albumin.
Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark.
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
100 µg 浓度为 1 mg/ml
Affinity purified
Fluorescein Antibody Biotin Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein coupled to sepharose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
多克隆
IgG
Abpromise™承诺保证使用ab6655于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Dot blot | Use at an assay dependent concentration. | |
| ELISA | 1/10000 - 1/50000. | |
| IHC-P | 1/1000 - 1/5000. PubMed: 26838601 | |
| IHC-Fr | 1/1000 - 1/5000. | |
| Immunomicroscopy | Use at an assay dependent concentration. | |
| In situ hybridization | Use at an assay dependent concentration. | |
| WB | 1/2000 - 1/10000. |
FI antibody
FITC antibody
fluorescein isothyocianate antibody
fluorescyl antibody
Western blot - Biotin Anti-Fluorescein antibody (ab6655)
Biotin Anti-Fluorescein antibody (ab6655) at 1/1000 dilution + BSA conjugated Fluorescein.
Secondary
Peroxidase conjugated Streptavidin secondary antibody at 1/40000 dilution
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Fluorescein antibody (ab6655)Cropped image from Long et al, Nature Communications, 7, 10354, Fig. 7a.; doi: 10.1038/ncomms10354 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Immunohistochemical analysis of chicken embryo neuroepithelium labelling fluorescein-tagged morpholinos with ab6655. Samples were fixed in 4% paraformaldehyde at 4°C for 1 hour.
