FITC荧光Anti-GFP抗体
参阅全部 GFP 一抗
FITC荧光山羊多克隆抗体to GFP
Goat
FITC. Ex: 493nm, Em: 528nm
No reaction was observed against Human, Mouse and Rat Serum Proteins.
适用于: IHC-FoFr, IHC-Fr, WB, ICC/IFmore details
与反应: Species independent
Recombinant full length protein corresponding to GFP aa 1-246.
Sequence:
MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTT GKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFF KDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNV YIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHY LSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
Database link: P42212
WB: Recombinant A. victoria GFP protein.
Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation. Fluorescein conjugated anti-GFP was assayed by immunofluorescence microscopy on prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and was shown to detect GFP containing inserts. Significant amplification of signal was detected using fluorochrome conjugated anti-GFP relative to the fluorescence of GFP alone.
In case of unexpected background, use pre-adsorbed secondary antibodies.
Fluorescein isothiocyanate (FITC) (MW 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.5 moles FITC per mole of Goat IgG
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
BSA Immunoglobulin and Protease free
浓度
100 µg 浓度为 1 mg/ml
100 µg 浓度为 1 mg/ml
Affinity purified
GFP Fluorescein Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation. Fluorescein conjugated anti-GFP was assayed by immunofluorescence microscopy on prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and was shown to detect GFP containing inserts. Significant amplification of signal was detected using fluorochrome conjugated anti-GFP relative to the fluorescence of GFP alone.
多克隆
IgG
Abpromise™承诺保证使用ab6662于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-FoFr | (1) | 1/250. |
| IHC-Fr | (5) | Use at an assay dependent concentration. |
| WB | (1) | 1/10000. |
| ICC/IF | (4) | 1/500 - 1/2500. |
GFP antibody
Green fluorescent protein antibody
Western blot - FITC Anti-GFP antibody (ab6662)
FITC Anti-GFP antibody (ab6662) + Recombinant A. victoria GFP protein (ab84191)
Secondary
Fluorescein goat secondary antibody for 60 minutes at RT at 1/1000 dilution
Block: Blocking buffer for 30 minutes at RT.
Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (ab6662)Kim at el PLoS Genet. 2018 Feb 8;14(2):e1007204. doi: 10.1371/journal.pgen.1007204. eCollection 2018 Feb. Fig 4. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Oscillation of Cyclin E and E2F target gene expression is deregulated in de2f1b mutant salivary glands.
Salivary glands of control and de2f1b mutant early (80–85 hr AEL) third instar larvae expressing PCNA-GFP (green, ab6662) are stained with anti-dE2F1 (red). The region where high PCNA-GFP is observed with low dE2F1 is marked by an asterisk.
For Immunostaining, third instar imaginal discs and salivary glands were dissected in PBS and immediately fixed in 4% formaldehyde in PBS for 20 minutes at room temperature with the exception of tissues subjected to anti-dE2F1 staining that were fixed for 30 minutes on ice. Fixed tissues were then washed with 0.3% PBST (0.3% TritonX-100 in 1XPBS) and 0.1% PBST (0.1% TritonX-100 in 1XPBS). Samples were incubated with appropriate amount of primary antibody in 0.1% PBST and 1%BSA overnight. Samples were then washed with 0.1% PBST, incubated in secondary antibody in 0.1% PBST and 1% BSA for 2 hours, followed by several washes in 0.1% PBST prior to mounting.
Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (ab6662)This image is courtesy of an Abreview submitted by Dr Joshua Breunig
ab6662 staining mouse brain tissue sections (inducible GFP reporter) by IHC-Fr.
The tissue was paraformaldehyde fixed and blocked with serum and then incubated with the antibody at a 1/1000 dilution for 1 hour.
Staining is shown in the left hand panel. The middle panel shows staining with a rabbit anti-GFP antibody and the right hand panel shows the merged images (plus DAPI).
ab6662 gives no noticable background and it is found that when viewing on an epifluorescent the exposure time is significantly reduced.
Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (ab6662)
Immunofluorescence Microscopy using ab6662.
Tissue: Sf-1:Cre mice crossed to the Z/EG reporter line. Mouse brain (coronal view, 20X magnification).
Fixation: 4% PFA/PBS with o/n fixation, and subsequently transferred to a 30% sucrose solution.
Antigen retrieval: Frozen in OCT freezing medium (Sakura) and cryostat sectioned at 40 microns.
Goat anti-GFP was used at 1/500 dilution in free floating imunnohistochemistry to detect GFP.
Secondary antibody: Fluorchrome conjugated Anti-goat IgG secondary antibody was used for detection at 1:500 at 1/10,000 for 45 minutes at RT.
Localization: Sf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus.
Staining: eGFP as green fluorescent signal and sections were counterstained with DAPI.
Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (ab6662)
These pictures show confocal immunofluorescence using GFP-expressing glial cells (green) transplanted into the lesioned rat spinal cord.
Detected using ab6662 and a standard FITC filter set. Axons are labeled red by an antibody to neurofilament-200 and a rhodamine secondary antibody. The upper panel shows the centre of the transplant site at low power. Numerous GFP-positive cells can be seen mingling with axons. The lower panel shows, at high power in a single optical section, how ab6662 reveals the morphology of the transplanted cells to such an extent that their close interactions with axons are obvious - the cell depicted can be seen wrapping around a neurofilament-200 positive axon.
These images were kindly supplied as part of the review submitted by Andrew Toft.
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