Texas Red ® Anti-Biotin抗体
参阅全部 Biotin 一抗
Texas Red ®山羊多克隆抗体to生物素
Goat
Texas Red ®. Ex: 596nm, Em: 620nm
适用于: WBmore details
与反应: Species independent
Chemical/ Small Molecule conjugated to KLH.
Texas Red™ is a registered trademark of Molecular Probes Inc.
Texas Red™ Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 2.5 moles Texas Red™ per mole of Goat IgG.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
100 µg 浓度为 1 mg/ml
Affinity purified
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.
多克隆
IgG
Abpromise™承诺保证使用ab6653于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use at an assay dependent concentration. |
Vitamin B4 antibody
Western blot - Texas Red ® Anti-Biotin antibody (ab6653)
Texas Red ® Anti-Biotin antibody (ab6653) at 1/1000 dilution (60 minutes at room temperature) + Biotin conjugated Guinea Pig IgG separated by SDS-PAGE at 0.05 µg
Observed band size: 25,55 kDawhy is the actual band size different from the predicted?
Blocking Buffer for Fluorescent Western Blotting for 30 min at room temperature.
