山羊F(ab)抗小鼠IgG H&L
参阅全部 IgG 二抗
Goat
Mouse
适用于: WB, ICC/IF, IHC, Dot blot, ELISAmore details
Mouse IgG whole molecule
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
1 mg 浓度为 1 mg/ml
Affinity purified
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
多克隆
IgG
Abpromise™承诺保证使用ab6668于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/2000 - 1/10000. | |
| ICC/IF | 1/1000 - 1/5000. | |
| IHC | 1/1000 - 1/5000. | |
| Dot blot | Use at an assay dependent concentration. | |
| ELISA | 1/15000. |
SDS-PAGE - Goat F(ab) Anti-Mouse IgG H&L (ab6668)
SDS-PAGE results of Goat Fab Anti-Mouse IgG (H&L) ab6668.
Lane 1: Non-reduced Goat Fab Anti-Mouse.
Lane 2: Opal Prestained Ladder.
Lane 3: Reduced Goat Fab Anti-Mouse.
Load: 1.0 µg.
Coomassie stained and imaged.
Dot Blot - Goat F(ab) Anti-Mouse IgG H&L (ab6668)
Dot Blot results of Goat Fab Anti-Mouse IgG Antibody ab6668.
Dots are Mouse IgG at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng.
Blocked for 30 min at RT.
Primary Antibody: Goat Fab Anti-Mouse IgG at 1 µg/mL for 1 hr at RT.
Secondary Antibody: Donkey Anti-Goat IgG HRP at 1:40,000 for 30 min at RT.
Imaged with BioRad ChemiDoc, Chemi filter.
