Anti-Fluorescein抗体
参阅全部 Fluorescein 一抗
山羊多克隆抗体to Fluorescein
Goat
适用于: ELISA, WBmore details
与反应: Species independent
Chemical/ Small Molecule corresponding to Fluorescein. Fluorescein conjugated to Goat IgG
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
100 µg 浓度为 1 mg/ml
100 µg 浓度为 1.1 mg/ml
Immunogen affinity purified
Fluorescein Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein conjugated Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
多克隆
IgG
Abpromise™承诺保证使用ab6644于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA | 1/5000 - 1/25000. | |
| WB | 1/500 - 1/3000. |
FI antibody
FITC antibody
fluorescein isothyocianate antibody
fluorescyl antibody
Western blot - Anti-Fluorescein antibody (ab6644)
Anti-Fluorescein antibody (ab6644) at 1/1000 dilution + BSA conjugated Fluorescein at 0.05 µg
Secondary
Goat secondary antibody (HRP) at 1/40000 dilution
Observed band size: 67 kDawhy is the actual band size different from the predicted?
Primary antibody incubated overnight at 4°C.
Secondary antibody incubated for 30 minutes at room temperature.
