Alkaline Phosphatase Anti-GFP抗体
参阅全部 GFP 一抗
Alkaline Phosphatase山羊多克隆抗体to GFP
Goat
Alkaline Phosphatase
Antibody recognizes wild type, recombinant and enhanced forms of GFP. Immunoblot shows a 42 kDa band when reacted with GFP on a Western blot. No reaction was observed against human, mouse and rat serum proteins.
适用于: WB, ELISA, Dot blotmore details
与反应: Species independent
Recombinant full length protein corresponding to GFP aa 1-250.
Database link: P42212
Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation.
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
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Liquid
Shipped at 4°C. Store at +4°C. Do Not Freeze.
pH: 8
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine), 0.788% Tris HCl, 0.88% Sodium chloride, 0.00952% Magnesium chloride, 0.00136% Zinc chloride
浓度
100 µg 浓度为 1 mg/ml
Affinity purified
Anti-GFP Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation.
多克隆
IgG
Abpromise™承诺保证使用ab6661于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/500 - 1/2500. | |
| ELISA | 1/2000 - 1/8000. This product has been assayed against 1.0 µg of GFP in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. | |
| Dot blot | 1/1000. |
GFP antibody
Green fluorescent protein antibody
Western blot - Alkaline Phosphatase Anti-GFP antibody (ab6661)
Alkaline Phosphatase Anti-GFP antibody (ab6661) at 1/1000 dilution + GFP at 0.05 µg with Blocking buffer
Dot Blot - Alkaline Phosphatase Anti-GFP antibody (ab6661)
Dot blott analysis of ab6661 used in all lanes at a concentration of 1/1,000 incubated at room temperature for 1 hour.
Lane 1: 100 ng GFP protein
Lane 2: 33.33 ng GFP protein
Lane 3: 11.11 ng GFP protein
Lane 4: 3.70 ng GFP protein
Lane 5: 1.23 ng GFP protein
