Anti-CD62P antibody [AK-6]

• 产品名称

  Anti-CD62P抗体[AK-6]
  参阅全部 CD62P 一抗

• 描述

  小鼠单克隆抗体[AK-6] to CD62P

• 宿主

  Mouse

• 特异性

  MCA796 recognises the CD62P glycoprotein, a 140kD molecule expressed by activated platelets and endothelial cells.

• 经测试应用

  适用于: Flow Cyt, IHC-P, ICC/IF, IHC-Frmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• ICC/IF: Human whole blood Flow Cyt: Human whole blood IHC-P: Human bone marrow and human colon tissue

• 常规说明

  This product has switched from a hybridoma to recombinant production method on 22nd July 2021.

  This product is a recombinant monoclonal antibody, which offers several advantages including:

  For more information see here.

• - High batch-to-batch consistency and reproducibility

• - Improved sensitivity and specificity

• - Long-term security of supply

• - Animal-free production

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.2
  Preservative: 0.01% Sodium azide
  Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS

• 浓度

• 100 µg 浓度为 0.545 mg/ml

• 1 mg 浓度为 0.545 mg/ml

• 40 µg 浓度为 0.545 mg/ml

• 纯度

  Protein A purified

• 纯化说明

  Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.

• 克隆

  单克隆

• 克隆编号

  AK-6

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab6632于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 6403 Human

• Omim: 173610 Human

• SwissProt: P16109 Human

• Unigene: 73800 Human

• 别名

• CD62P antibody

• GMP 140 antibody

• GMP-140 antibody

• GMP140 antibody

• GMRP antibody

• Granule membrane protein 140 antibody

• Granulocyte membrane protein antibody

• GRMP antibody

• LECAM 3 antibody

• LECAM3 antibody

• Leukocyte endothelial cell adhesion molecule 3 antibody

• Leukocyte-endothelial cell adhesion molecule 3 antibody

• LYAM3_HUMAN antibody

• P Selectin antibody

• P-selectin antibody

• PADGEM antibody

• Platelet activation dependent granule-external membrane protein antibody

• Platelet alpha granule membrane protein antibody

• PSEL antibody

• Selectin P (granule membrane protein 140kDa, antigen CD62) antibody

• SELP antibody

• Antigen CD62 antibody

• CD62 antibody

• CD62 antigen-like family member P antibody

• 

  Flow Cytometry - Anti-CD62P antibody [AK-6] (ab6632)

  Flow cytometry staining of human whole blood with ab6632 (right) or mouse IgG1κ (ab170190) isotype (left). Red blood cells of 200 µl blood were lysed, then cells were incubated for 30 min on ice in 1x PBS containing 10 µg/ml human IgG and 10% normal goat serum to block Fc receptors and non-specific protein-protein interaction followed by the antibody (ab6632) or mouse IgG1κ (ab170190) isotype (1x106 in 100 µl; at 0.2 µg/ml) for 30 min on ice. The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor®488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min on ice. The cells were simultaneously stained with CD42a PE. Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on alive neutrophils.

   

• 

  Immunohistochemistry (Frozen sections) - Anti-CD62P antibody [AK-6] (ab6632)

  IHC image of CD62P staining in a section of frozen normal human colon* performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab6632, 0.01ug/ml for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  
  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [AK-6] (ab6632)

  IHC image of CD62P staining in a section of formalin-fixed paraffin-embedded normal human bone marrow* performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab6632, 10ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [AK-6] (ab6632)

  IHC image of CD62P staining in a section of formalin-fixed paraffin-embedded normal human colon* performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab6632, 10ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• 

  Immunocytochemistry/ Immunofluorescence - Anti-CD62P antibody [AK-6] (ab6632)

  ab6632 staining CD62P in Human whole blood. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab6632 at 10μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).