兔抗小鼠IgG H&L
参阅全部 IgG 二抗
Rabbit
Mouse
适用于: WB, IP, Immunodiffusion, Conjugation, ICC/IF, ELISA, IHC-P, IHC-Frmore details
Mouse IgG, whole molecule
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
1 mg 浓度为 2 mg/ml
Affinity purified
Anti-Mouse IgG (H&L) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose.
多克隆
Abpromise™承诺保证使用ab6709于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/2000 - 1/10000. | |
| IP | Use at an assay dependent concentration. | |
| Immunodiffusion | Use at an assay dependent concentration. | |
| Conjugation | Use at an assay dependent concentration. | |
| ICC/IF | 1/1000 - 1/5000. | |
| ELISA | 1/20000 - 1/400000. | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. |
ELISA - Rabbit Anti-Mouse IgG H&L (ab6709)
Sensitivity of ab6709 against mouse IgG.
The starting dilution of ab6709 was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. The blocking agent was 3% fish gelatin. The detection antibody was a Goat anti-Rabbit IgG Antibody Peroxidase conjugate. TMB color development.
