Anti-MHC class I antibody [OX18]

• 产品名称

  Anti-MHC class I抗体[OX18]
  参阅全部 MHC class I 一抗

• 描述

  小鼠单克隆抗体[OX18] to MHC class I

• 宿主

  Mouse

• 经测试应用

  适用于: IHC-Fr, Flow Cytmore details

• 种属反应性

  与反应: Rat
  预测可用于: Human

• 免疫原

  Full length protein. This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• IHC-Fr: Normal rat placenta tissue. Flow Cyt: Lewis rat splenocytes.

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.02% Sodium azide
  Constituents: PBS, 6.97% L-Arginine

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  OX18

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab6405于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 3105 Human

• Omim: 142800 Human

• SwissProt: P30443 Human

• 别名

• Aw 24 antibody

• Aw 68 antibody

• CLASS I HISTOCOMPATIBILITY ANTIGEN antibody

• H2 K1 antibody

• H2K antibody

• HLA A antibody

• HLA class I histocompatibility antigen A 1 alpha chain antibody

• HLA class I histocompatibility antigen A 11 alpha chain antibody

• HLA class I histocompatibility antigen A 2 alpha chain antibody

• HLA class I histocompatibility antigen A 24 alpha chain antibody

• HLA class I histocompatibility antigen A 3 alpha chain antibody

• HLA class I histocompatibility antigen A 30 alpha chain antibody

• HLA class I histocompatibility antigen A 32 alpha chain antibody

• HLA class I histocompatibility antigen A 68 alpha chain antibody

• HLAA antibody

• Leukocyte antigen class I A antibody

• Major histocompatibility complex class I A antibody

• MHC class I antigen A 1 antibody

• MHC class I antigen A*11 antibody

• MHC class I antigen A*2 antibody

• MHC class I antigen A*24 antibody

• MHC class I antigen A*3 antibody

• MHC class I antigen A*30 antibody

• MHC class I antigen A*32 antibody

• MHC class I antigen A*68 antibody

• MHC class I antigen HLA A heavy chain antibody

• MHC class I heavy chain H2 K antibody

• A 28 antibody

• A 9 antibody

• Antigen presenting molecule antibody

• 

  Flow Cytometry - Anti-MHC class I antibody [OX18] (ab6405)

  Lewis rat splenocytes stained with ab6405 (right) or mouse IgG1 kappa; (ab170190) isotype (left). Cells were incubated for 30 min on ice in 1x PBS containing 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab6405) or mouse IgG1 kappa; (ab170190) isotype (1x 106 in 100 µL at 0.2 µg/mL) for 30 min on ice. The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min on ice. The cells were simultaneously stained with CD3. Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on live single cells.

• 

  Immunohistochemistry (Frozen sections) - Anti-MHC class I antibody [OX18] (ab6405)

  IHC image of MHC Class I staining in a section of frozen normal rat placenta*.

  The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature.  The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab6405 (shown in green) at 1 μg/ml. The section was then incubated with ab150117 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 488, 1/1000)) presabsorbed for 1 hour at room temperature. The secondary-only control image is taken from an identical assay without primary antibody. The section was then mounted using Fluoromount® .

  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.

  *Tissue obtained from Charles River.