Anti-KPNA2抗体
参阅全部 KPNA2 一抗
山羊多克隆抗体to KPNA2
Goat
适用于: Flow Cyt, ICC/IF, IHC-P, WBmore details
与反应: Rat, Human
预测可用于: Mouse
Synthetic peptide corresponding to Human KPNA2 aa 518-529 (C terminal).
Sequence:
QVQDGAPGTFNF
Database link: P52292
WB: KNRK, Jurkat, CaCo-2, A549 and MCF7 cell lysate; ICC/IF: A549 and U2OS cells Flow Cyt: A549 cells
GenBank Accession Number – NP_002257.
The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination.
多克隆
IgG
Abpromise™承诺保证使用ab6036于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt | Use a concentration of 10 µg/ml. | |
| ICC/IF | Use a concentration of 10 µg/ml. | |
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 58 kDa). A 1 hour primary incubation is recommended for this product. |
Entrez Gene: 3838 Human
Entrez Gene: 16647 Mouse
Omim: 600685 Human
SwissProt: P52292 Human
SwissProt: P52293 Mouse
Unigene: 594238 Human
Unigene: 12508 Mouse
Unigene: 391577 Mouse
Unigene: 423000 Mouse
IMA1_HUMAN antibody
Importin alpha 1 antibody
Importin alpha 2 antibody
Importin alpha 2 subunit antibody
Importin alpha P1 antibody
Importin subunit alpha-1 antibody
IPO A1 antibody
IPOA 1 antibody
IPOA1 antibody
Karyopherin alpha 2 antibody
Karyopherin alpha 2 RAG cohort 1 antibody
Karyopherin alpha 2 subunit antibody
Karyopherin subunit alpha-2 antibody
KPNA2 antibody
KPNA2 protein antibody
Pendulin antibody
QIP 2 antibody
QIP2 antibody
RAG cohort 1 antibody
RAG cohort protein 1 antibody
RCH 1 antibody
RCH1 antibody
SRP 1 antibody
SRP1 alpha antibody
SRP1 antibody
SRP1-alpha antibody
SRP1alpha antibody
Western blot - Anti-KPNA2 antibody (ab6036)
Lanes 1-2 : Anti-KPNA2 antibody (ab6036) at 0.1 µg/ml
Lanes 3-5 : Anti-KPNA2 antibody (ab6036) at 0.03 µg/ml
Lane 1 : Jurkat cell lysate
Lane 2 : CaCo-2 cell lysate
Lane 3 : A549 cell lysate
Lane 4 : MCF7 cell lysate
Lane 5 : KNRK cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 58 kDa
Detected by chemiluminescence.
Flow Cytometry - Anti-KPNA2 antibody (ab6036)
Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-KPNA2 antibody (ab6036)
Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL).
Immunocytochemistry/ Immunofluorescence - Anti-KPNA2 antibody (ab6036)
Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL), showing cytoplasmic and ER/Golgi staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL).
