山羊F(ab')2抗兔IgG - H&L (Biotin),预吸附二抗
参阅全部 IgG 二抗
Goat
Rabbit
No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
适用于: ICC/IF, IHC-P, Dot blot, WB, ELISA, IHC, Immunomicroscopymore details
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheepmore details
Rabbit IgG whole molecule
Biotin
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
BSA is IgG and protease free
浓度
500 µg 浓度为 1 mg/ml
Affinity purified
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
多克隆
IgG
Abpromise™承诺保证使用ab6012于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | 1/1000 - 1/5000. | |
IHC-P | Use at an assay dependent dilution. PubMed: 21498392 | |
Dot blot | Use at an assay dependent dilution. | |
WB | 1/2000 - 1/10000. | |
ELISA | 1/20000 - 1/100000. | |
IHC | Use at an assay dependent dilution. | |
Immunomicroscopy | Use at an assay dependent dilution. |