山羊F(ab')2抗兔IgG Fc (Biotin)
参阅全部 IgG 二抗
Goat
Rabbit
No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Rabbit IgG F(ab).
适用于: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopy, IPmore details
Rabbit IgG F(c) fragment
Biotin
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
BSA is IgG and protease free
浓度
500 µg 浓度为 1 mg/ml
Affinity purified
F(ab') fragment biotin conjugated secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG F(ab’)2 molecule
多克隆
IgG
Abpromise™承诺保证使用ab6021于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | 1/1000 - 1/5000. | |
Dot blot | Use at an assay dependent dilution. | |
WB | 1/2000 - 1/10000. | |
ELISA | 1/20000 - 1/100000. | |
IHC | Use at an assay dependent dilution. | |
Immunomicroscopy | Use at an assay dependent dilution. | |
IP | Use at an assay dependent dilution. |