Recombinant Furin protein

• 产品名称

  重组Furin蛋白
  参阅全部 Furin 蛋白酶

• 表达系统

  Mammalian

• Accession

  P09958

• 蛋白长度

  Full length protein

• 无动物成分

  No

• 性质

  Recombinant

• 
  

• 预测分子量

  87 kDa

• 氨基酸

  1 to 713

• 应用

  Western blot

• 形式

  Liquid

• 补充说明

  This 81 kDa recombinant protein was obtained from cytoplasmic extracts by centrifugation and lysis of BSC-40 cells infected with recombinant vaccinia virus (VV:hFUR713t). The preparation was subjected to UV irradiation for 5 minutes to inactivate any residual virus. Activity of 1 U/µl, where 1 U (a unit of activity) is defined as the amount of furin that can release 1 pmol of AMC from the fluorogenic peptide pERTKR-MCA in 1 min.

  
  

  Furin is a membrane-associated, calcium-dependent, serine protease that belongs to the subtilisin-like prohormone convertase (PC) family. Members of this family of cellular enzymes cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. Furin and other PC family members share structural similarities which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. Furin is the only known PC to possess a transmembrane domain. Cleavage of target proteins occurs at the carboxyl-terminus of the furin consensus sequence, RX(K/R)R. It has been shown that the acidic peptide sequence, C771PSDSEEDEG780, localizes furin to the trans-Golgi-network. Phosphorylation of serine residues within this region modulates intracellular routing of furin protein. An additional signaling domain includes the tetrapeptide sequence, Y759KGL762, which directs internalization from the cell surface.

• 稳定性和存储

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  Preservative: 0.05% Sodium azide
  Constituent: 0.1% BSA

  This product is an active protein and may elicit a biological response in vivo, handle with caution.

别名

• Dibasic processing enzyme

• Dibasic-processing enzyme

• FES upstream region

• FUR

• FURIN

• Furin membrane associated receptor protein

• FURIN_HUMAN

• PACE

• Paired basic amino acid residue cleaving enzyme

• Paired basic amino acid residue-cleaving enzyme

• PCSK3

• Proprotein convertase subtilisin/kexin type 3

• SPC1