山羊F(ab')2抗小鼠IgG - (Fab)'2 (PE),预吸附二抗
参阅全部 IgG 二抗
Goat
Mouse
No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(c) or Bovine, Horse or Human Serum Proteins.
适用于: ICC/IF, Immunomicroscopy, Flow Cyt, WB, Dot blotmore details
Cow, Horse, Human, Rabbit, Rat, Sheepmore details
Mouse IgG F(ab)2 fragment
PE. Ex: 488nm, Em: 575nm
Liquid
Shipped at 4°C. Store at +4°C.
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
BSA is IgG and protease free
浓度
批次浓度范围 500 µl 浓度为 0.5 - 1 mg/ml
Affinity purified
F(ab')2 fragment PE conjugated Anti-Mouse IgG F(ab')2 was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
R-Phycoerythrin (R-PE) (Molecular Weight 240,000 daltons) from seaweed Absorption Wavelength: 490 nm, 545 nm and 565 nm Emission Wavelength: 580 nm OD566nm/OD280nm: 2.6 Phycobiliprotein Concentration: 0.5 mg/ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound).
多克隆
IgG
Abpromise™承诺保证使用ab5889于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | 1/100 - 1/250. | |
Immunomicroscopy | Use at an assay dependent concentration. Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. | |
Flow Cyt | 1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. | |
WB | 1/100 - 1/500. Predicted molecular weight: 36 kDa. | |
Dot blot | Use at an assay dependent concentration. |