Anti-hnRNP A1 antibody [9H10]

• 产品名称

  Anti-hnRNP A1抗体[9H10]
  参阅全部 hnRNP A1 一抗

• 描述

  小鼠单克隆抗体[9H10] to hnRNP A1

• 宿主

  Mouse

• 经测试应用

  适用于: Flow Cyt, IHC-P, ELISA, WB, IP, ICC/IFmore details

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Full length hnRNPA1 native protein (partially purified) from HeLa cells (Human).

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.1% Sodium azide
  Constituent: PBS

• 浓度

• 100 µl 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 纯化说明

  Purified from tissue culture supernatant.

• 克隆

  单克隆

• 克隆编号

  9H10

• 骨髓瘤

  Sp2/0

• 同种型

  IgG2b

The Abpromise guarantee

Abpromise™承诺保证使用ab5832于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 3178 Human

• Entrez Gene: 15382 Mouse

• Omim: 164017 Human

• SwissProt: P09651 Human

• SwissProt: P49312 Mouse

• Unigene: 546261 Human

• Unigene: 655424 Human

• Unigene: 237064 Mouse

• Unigene: 432031 Mouse

别名

• HNRNPA 1 antibody

• Helix destabilizing protein antibody

• Helix-destabilizing protein antibody

• Heterogeneous nuclear ribonucleoprotein A1 antibody

• Heterogeneous nuclear ribonucleoprotein A1B protein antibody

• Heterogeneous nuclear ribonucleoprotein B2 protein antibody

• Heterogeneous nuclear ribonucleoprotein core protein A1 antibody

• hnRNP A1 antibody

• hnRNP core protein A1 antibody

• HNRNPA1 antibody

• HNRPA1 antibody

• MGC102835 antibody

• Nuclear ribonucleoprotein particle A1 protein antibody

• ROA1_HUMAN antibody

• Single strand DNA binding protein UP1 antibody

• Single strand RNA binding protein antibody

• Single-strand RNA-binding protein antibody

• 

  Western blot - Anti-hnRNP A1 antibody [9H10] (ab5832)

  All lanes : Anti-hnRNP A1 antibody [9H10] (ab5832) at 1 µg/ml
  
  Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
  Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
  Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 38 kDa
  Observed band size: 37 kDawhy is the actual band size different from the predicted?
  Additional bands at: 43 kDa. We are unsure as to the identity of these extra bands.
  
  
  Exposure time: 30 seconds
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [9H10] (ab5832)

  Ab5832 staining human lung. Staining is localized to the nucleus.
  Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
  Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer, citrate pH 6.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

• 

  Western blot - Anti-hnRNP A1 antibody [9H10] (ab5832)This image is courtesy of an anonymous Abreview.

  All lanes : Anti-hnRNP A1 antibody [9H10] (ab5832) at 1/1000 dilution
  
  Lane 1 : Mouse NSC34 whole cell lysate with control siRNA
  Lane 2 : Mouse NSC34 whole cell lysate with hnRNP A1 siRNA
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  All lanes : IRDye® 700DX-conjugated Donkey anti-Mouse IgG at 1/3000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size: 38 kDa
  
  
  Exposure time: 1 minute
  
  
  Knockdown for 72 hours.

  See Abreview

• 

  Flow Cytometry - Anti-hnRNP A1 antibody [9H10] (ab5832)

  Overlay histogram showing Jurkat cells stained with ab5832 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab5832, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
  
  

• 

  Immunoprecipitation - Anti-hnRNP A1 antibody [9H10] (ab5832)

  hnRNP A1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to hnRNP A1 (ab5832) and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
  The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
  Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab5832.
  Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
  Band: 37kDa: hnRNP A1; 42kDa:We are unsure as to the identity of this extra band.