Anti-ERK5 (phospho T219 + Y221)抗体
参阅全部 ERK5 一抗
兔多克隆抗体to ERK5 (phospho T219 + Y221)
Rabbit
Some cross-reactivity is observed with endogenous ERK1 and 2 (44 and 42 kDa, respectively) due to the high levels of expression and activation of this protein typically observed with most cell types.
适用于: WBmore details
与反应: Human
预测可用于: Mouse
Synthetic peptide corresponding to ERK5 (phospho T219 + Y221).
WB: HEK293 cells transiently co-transfected with plasmids expressing ERK5 kinase domain (ERK5kin) and constitutively activated MEK5 (MEK5D-D).
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Immunogen affinity purified
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated ERK5. The final product is generated by affinity chromatography using an ERK5-derived peptide that is phosphorylated at threonine 219 and tyrosine 221.
多克隆
IgG
Abpromise™承诺保证使用ab5686于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) | 1/1000. Predicted molecular weight: 88 kDa. Due to the low abundance and low levels of activation of endogenous ERK5, over-expression or immunoprecipitation may be required. |
Entrez Gene: 5598 Human
Entrez Gene: 23939 Mouse
Omim: 602521 Human
SwissProt: Q13164 Human
SwissProt: Q9WVS8 Mouse
Unigene: 150136 Human
Unigene: 38172 Mouse
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Western blot - Anti-ERK5 (phospho T219 + Y221) antibody (ab5686)
Peptide Competition:
Extracts prepared from HEK293 cells transiently transfected with plasmids expressing ERK5 kinase domain (ERK5kin) and constitutively activated MEK5D-D were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA TBST buffer overnight at 4oC, then were incubated with the ab5686 antibody for two hours at room temperature in a 3% BSATBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine-containing peptide (3), a generic phosphotyrosine-containing peptide (4), the phosphopeptide derived from the corresponding region of ERK1&2 (5), or, the phosphopeptide immunogen (6). After washing, membranes were incubated with goat F(ab’ 2 anti-rabbit IgG alkaline phosphatase conjugate and bands were detected using the Tropix WesternStarTM detection method. The data show that while there is some cross-reactivity with ERK1&
