Anti-Hsp27 (phospho S15) antibody

• 产品名称

  Anti-Hsp27 (phospho S15)抗体
  参阅全部 Hsp27 一抗

• 描述

  兔多克隆抗体to Hsp27 (phospho S15)

• 宿主

  Rabbit

• 特异性

  ab5581 detects phosphorylated heat shock protein 27(hsp27) from rat and Human tissues.

• 经测试应用

  适用于: WB, ICC/IF, IPmore details

• 种属反应性

  与反应: Human
  预测可用于: Cow, Dog, Pig

• 免疫原

  Synthetic peptide corresponding to Human Hsp27 aa 10-21 (phospho S15).
  Sequence:

  LLRGPSWDPFRC

  
  (Peptide available as ab41772)

  Run BLAST with Run BLAST with 

• 阳性对照

• ICC/IF: HeLa cells. WB: HeLa cell lysate. IP: HeLa cell lysate.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituents: 0.1% BSA, 99% PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Immunogen affinity purified

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab5581于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 516099 Cow

• Entrez Gene: 3315 Human

• Entrez Gene: 493184 Pig

• Omim: 602195 Human

• SwissProt: Q3T149 Cow

• SwissProt: P04792 Human

• SwissProt: Q5S1U1 Pig

• Unigene: 520973 Human

• 别名

• DKFZp586P1322 antibody

• epididymis secretory protein Li 102 antibody

• Estrogen regulated 24 kDa protein antibody

• Estrogen-regulated 24 kDa protein antibody

• Heat shock 25kDa protein 1 antibody

• Heat shock 27 kDa protein antibody

• Heat shock 27kD protein 1 antibody

• Heat shock 27kDa protein 1 antibody

• Heat shock 28kDa protein 1 antibody

• Heat Shock Protein 27 antibody

• Heat shock protein beta 1 antibody

• Heat shock protein beta-1 antibody

• heat shock protein family B (small) member 1 antibody

• HEL-S-102 antibody

• HMN2B antibody

• HS.76067 antibody

• Hsp 25 antibody

• HSP 27 antibody

• Hsp 28 antibody

• Hsp B1 antibody

• Hsp25 antibody

• HSP27 antibody

• Hsp28 antibody

• HspB1 antibody

• HSPB1_HUMAN antibody

• SRP27 antibody

• Stress responsive protein 27 antibody

• Stress-responsive protein 27 antibody

• Heat shock 27kDa protein antibody

• 28 kDa heat shock protein antibody

• CMT2F antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S15) antibody (ab5581)

  Immunocytochemistry analysis of Hsp27 using ab5581 at 2µg/mL concentration shows staining in 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized HeLa Cells treated with Anisomysin. The secondary antibody was Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate. Hsp27 (green), F-Actin staining with Alexa Fluor® 555 Rhodamine Phalloidin (red) and nuclei with SlowFade® Gold Antifade Mountant with DAPI (blue) is shown. Negative control has no primary antibody.

• 

  Western blot - Anti-Hsp27 (phospho S15) antibody (ab5581)

  All lanes : Anti-Hsp27 (phospho S15) antibody (ab5581) at 1/500 dilution
  
  Lane 1 : HeLa cell lysate - untreated
  Lane 2 : HeLa cell lysate - treated with 10nM Anisomycin
  
  Lysates/proteins at 50 µg per lane.
  
  Secondary
  All lanes : HRP-conjugated goat anti-rabbit IgG at 1/20000 dilution
  
  Predicted band size: 23 kDa
  
  

• 

  Immunoprecipitation - Anti-Hsp27 (phospho S15) antibody (ab5581)

  Immunoprecipitation of Hsp27 (phospho S15) was performed on HeLa cells treated with 10uM Anisomysin for 30 minutes. Antigen-antibody complexes were formed by incubating 500ug of whole cell lysate with 3ug of ab5581 overnight on a rocking platform at 4°C. The immune complexes were captured on 50ul Protein A/G Agarose, washed extensively, and eluted with Lane Marker Reducing Sample Buffer. Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with ab5581 at 1:500 overnight rotating at 4°C, washed with TBST, and probed with Clean-Blot IP Detection Reagent at 1/1000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S15) antibody (ab5581)

  Immunocytochemistry/Immunofluorescence analysis of Hsp27 (phospho S15) (green) in HeLa cells either untreated (left) or treated with 10uM Anisomysin (right) for 30 minutes. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% blocker BSA for 15 minutes at room temperature. Cells were incubated with ab5581 at 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (1:400) for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye. 20X magnification.