Anti-VEGF Receptor 2 (phospho Y1214)抗体
参阅全部 VEGF Receptor 2 一抗
兔多克隆抗体to VEGF Receptor 2 (phospho Y1214)
Rabbit
VEGFR3 has not been tested, but is expected to react.
适用于: WBmore details
与反应: Human, Recombinant fragment
预测可用于: Rat
Synthetic peptide corresponding to Human VEGF Receptor 2 (phospho Y1214).
WB: NIH 3T3 cells transfected with full length, wild-type human VEGFR 2 and Porcine Aortic Endothelial cells transfected with a chimeric receptor consisting of the extracellular domain of the CSF 1 receptor coupled to the transmembrane and cytoplasmic domains of the mouse VEGFR 2. HT-29 Serum Starved followed by treatment for 10 minutes with 100 ng/mL of SCF, Caco-2 Serum Starved followed by treatment for 10 minutes with 100 ng/mL of SCF.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
BSA is IgG and protease free
浓度
50 µl 浓度为 0.5 mg/ml
Immunogen affinity purified
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated VEGFR 2 protein. The final product is generated by affinity chromatography using a VEGFR 2 derived peptide that is phosphorylated at Tyrosine 1214.
多克隆
IgG
Abpromise™承诺保证使用ab5475于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 0.35 - 1 µg/ml. Detects a band of approximately 200 kDa. |
Entrez Gene: 3791 Human
Omim: 191306 Human
SwissProt: P35968 Human
Unigene: 479756 Human
Unigene: 88869 Rat
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Fetal liver kinase 1 antibody
FLK-1 antibody
FLK1 antibody
FLK1, mouse, homolog of antibody
Kdr antibody
Kinase insert domain receptor (a type III receptor tyrosine kinase) antibody
Kinase insert domain receptor antibody
KRD1 antibody
Ly73 antibody
Protein tyrosine kinase receptor FLK1 antibody
Protein-tyrosine kinase receptor flk-1 antibody
soluble VEGFR2 antibody
Tyrosine kinase growth factor receptor antibody
Vascular endothelial growth factor receptor 2 antibody
VEGFR 2 antibody
VEGFR antibody
VEGFR-2 antibody
VEGFR2 antibody
VGFR2_HUMAN antibody
CD309 antibody
CD309 antigen antibody
EC 2.7.10.1 antibody
Western blot - Anti-VEGF Receptor 2 (phospho Y1214) antibody (ab5475)
Peptide Competition and Stimulation: Extracts prepared from CSF-1 stimulated (1-4, 6) or unstimulated (5) PAE cells transfected with a chimeric CSF-1/VEGFR2 receptor were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50 µg/mL ab5475 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 5, 6), the nonphosphopeptide corresponding to the immunogen (2), a generic phosphotyrosine containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab5475 blocks the antibody signal, and the stimulation of the phospho signal after stimulating ligand is added (CSF-1 for the chimeric r
Western blot - Anti-VEGF Receptor 2 (phospho Y1214) antibody (ab5475)
All lanes : Anti-VEGF Receptor 2 (phospho Y1214) antibody (ab5475) at 1/1000 dilution
Lane 1 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate
Lane 2 : HT-29 Serum Starved followed by treatment for 10 minutes with 100 ng/mL of SCF
Lane 3 : Caco-2 (Human colorectal adenocarcinoma cell line) whole cell lysate
Lane 4 : Caco-2 Serum Starved followed by treatment for 10 minutes with 100 ng/mL of SCF
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/5000 dilution
Observed band size: ~151 kDawhy is the actual band size different from the predicted?
Modulation of expression of target protein by cell treatment to demonstrate antibody specificity.
The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate
