Anti-Integrin beta 1 (phospho T788 + T789)抗体
参阅全部 Integrin beta 1 一抗
兔多克隆抗体to Integrin beta 1 (phospho T788 + T789)
Rabbit
适用于: ICC, WBmore details
与反应: Human
预测可用于: Mouse, Rat, Chicken
Synthetic peptide corresponding to Human Integrin beta 1 (phospho T788 + T789).
Database link: P05558
WB: HeLa cells in mitosis. ICC: THP-1 cells.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
BSA is IgG and protease free
浓度
批次浓度范围 100 µl 浓度为 0.5 - 0.8 mg/ml
Immunogen affinity purified
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that isb reactive with non-phosphorylated Integrin beta 1 receptor protein. The final product is generated by affinity chromatography using an Integrin receptor-derived peptide that is phosphorylated at threonine 788 and threonine 789.
多克隆
IgG
Abpromise™承诺保证使用ab5189于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | 1/250. | |
| WB | (2) | Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 88 kDa. |
Entrez Gene: 374058 Chicken
Entrez Gene: 3688 Human
Entrez Gene: 16412 Mouse
Omim: 135630 Human
SwissProt: P07228 Chicken
SwissProt: P05556 Human
SwissProt: P09055 Mouse
Unigene: 643813 Human
Unigene: 263396 Mouse
Unigene: 25733 Rat
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Western blot - Anti-Integrin beta 1 (phospho T788 + T789) antibody (ab5189)
Peptide Competition: HeLa extracts of mitotic cells generated by treatment with taxol, were resolved by SDS-PAGE on a 10% Tris-glycine gel. The proteins then were transferred to nitrocellulose and incubated with 0.50 ab5189 antibody, following prior incubation with: (1) the phosphopeptide immunogen, (2) the non-phosphorylated peptide corresponding to the phosphopeptide, (3) a generic phosphothreonine-containing peptide, and (4) no peptide. After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar detection method. The data show that only the phosphopeptide corresponding to this site blocks the antibody signal, demonstrating the specificity of the Integrin beta 1 receptor antibody for this phosphorylated residue. Peptide Competition: HeLa extracts of mitotic cells generated by treatment with taxol, were resolved by SDS-PAGE on a 10% Tris-glycine gel. The proteins then were transferred to nit
Immunocytochemistry - Anti-Integrin beta 1 (phospho T788 + T789) antibody (ab5189)
THP-1 cells stained for Integrin beta 1 (green) using ab5189 at 1/250 dilution in ICC/IF. Followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1/300 dilution. Panel d represents the merged image showing localization in the membrane. Panel e is untreated cell with less signal. Panel f represents control cells with no primary antibody to assess background.
