Anti-SIAH1抗体
参阅全部 SIAH1 一抗
山羊多克隆抗体to SIAH1
Goat
适用于: WB, ICC/IFmore details
与反应: Rat, Human
预测可用于: Mouse, Cow, Dog
Synthetic peptide corresponding to Human SIAH1 aa 2-16 (N terminal).
Sequence:
SRQTATALPTGTSKC
WB: Rat liver lysate, human liver lysate. ICC/IF- U2OS cells and HepG2 cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris, 0.5% BSA
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
多克隆
IgG
Abpromise™承诺保证使用ab2237于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) | Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 31 kDa. 1 hour primary incubation at room temperature is recommended for this product. |
| ICC/IF | Use a concentration of 10 µg/ml. |
hide
Unigene: 713615 Human
Unigene: 73937 Rat
Entrez Gene: 6477 Human
Entrez Gene: 20437 Mouse
Omim: 602212 Human
SwissProt: Q8IUQ4 Human
SwissProt: P61092 Mouse
HUMSIAH antibody
Seven in absentia homolog 1 (Drosophila) antibody
Seven in absentia homolog 1 antibody
Siah 1 antibody
Siah 1a antibody
Siah E3 ubiquitin protein ligase 1 antibody
Siah-1 antibody
Siah-1a antibody
Siah1 antibody
SIAH1_HUMAN antibody
SIAH1A antibody
Ubiquitin ligase SIAH1 antibody
E3 ubiquitin-protein ligase SIAH1 antibody
FLJ08065 antibody
hSIAH1 antibody
Western blot - Anti-SIAH1 antibody (ab2237)
Anti-SIAH1 antibody (ab2237) at 1 mg/ml + Human liver lysate at 35 µg
Predicted band size: 31 kDa
Observed band size: 37 kDawhy is the actual band size different from the predicted?
Primary incubation 1 hour at room temperature. Detected by chemiluminescence. RIPA buffer was used.
Western blot - Anti-SIAH1 antibody (ab2237)
Anti-SIAH1 antibody (ab2237) at 1 µg/ml + Rat liver lysate at 35 µg
Predicted band size: 31 kDa
Observed band size: 30, 37 kDawhy is the actual band size different from the predicted?
Primary incubation 1 hour at room temperature. Detected by chemiluminescence. RIPA buffer was used.
Immunocytochemistry/ Immunofluorescence - Anti-SIAH1 antibody (ab2237)
Immunofluorescence analysis of paraformaldehyde fixed, 0.15% Triton permeabilized HepG2 (human liver hepatocellular carcinoma cell) cells labelling SIAH1 with primary anti-SIAH1 antibody (ab2237) at 10ug/ml for 1 hour, followed by Alexa Fluor 488 secondary antibody at 2ug/ml. Image showing nuclear and cytoplasmic staining. The nuclear counterstain is DAPI (blue).
Negative control: Unimmunized goat IgG at 10ug/ml followed by Alexa Fluor 488 secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-SIAH1 antibody (ab2237)
Immunofluorescence analysis of paraformaldehyde fixed, 0.15% Triton permeabilized U2OS (human bone osteosarcoma epithelial cell) cells labelling SIAH1 with primary anti-SIAH1 antibody (ab2237) at 10ug/ml for 1 hour, followed by Alexa Fluor 488 secondary antibody at 2ug/ml. Image showing nuclear and cytoplasmic staining. The nuclear counterstain is DAPI (blue).
Negative control: Unimmunized goat IgG at 10ug/ml followed by Alexa Fluor 488 secondary antibody.
