Anti-CDYL抗体
参阅全部 CDYL 一抗
兔多克隆抗体to CDYL
Rabbit
适用于: IHC-P, ChIP, WB, ChIP-sequencingmore details
与反应: Mouse, Rat, Human, African green monkey
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
This antibody gave a positive signal in COS7 cells transfected with Human CDYL (Whole Cell Lysate)
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab5188于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| ChIP | (1) | Use at an assay dependent concentration. PubMed: 22009739 |
| WB | (1) | 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 66 kDa). |
| ChIP-sequencing | Use at an assay dependent concentration. PubMed: 22009739 |
Entrez Gene: 9425 Human
Entrez Gene: 12593 Mouse
Omim: 603778 Human
SwissProt: Q9Y232 Human
SwissProt: Q9WTK2 Mouse
Unigene: 269092 Human
Unigene: 29002 Mouse
Unigene: 146844 Rat
CDY-like antibody
CDY-like autosomal antibody
Cdyl antibody
CDYL: chromodomain protein Y-like antibody
CDYL1 antibody
CDYL1_HUMAN antibody
chromodomain protein Y chromosome-like 6 antibody
Chromodomain protein Y like antibody
Chromodomain Y-like protein antibody
DKFZP586C1622 antibody
MGC131936 antibody
OTTHUMP00000015984 antibody
OTTHUMP00000211185 antibody
OTTHUMP00000211186 antibody
OTTHUMP00000211189 antibody
testis-specific chromodomain Y-like protein antibody
Weakly similar to enoyl-CoA hydratase [H.sapiens] antibody
Western blot - Anti-CDYL antibody (ab5188)
All lanes : Anti-CDYL antibody (ab5188) at 1 µg/ml
Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate - Untransfected
Lane 2 : COS7 CDYL (Human) Transfected Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 66 kDa
Observed band size: 80 kDawhy is the actual band size different from the predicted?
Additional bands at: 60 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
Western blot - Anti-CDYL antibody (ab5188)Cecile Lestrat, Saadi Khochbin lab, Grenoble, France
All lanes : Anti-CDYL antibody (ab5188) at 1/1000 dilution
Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
Lane 2 : COS7 cells transfected with HA-CDYL-FLAG plasmid
Predicted band size: 66 kDa
Observed band size: 83 kDawhy is the actual band size different from the predicted?
Rabbit polyclonal to CDYL (ab5188) at 1/1000.
Left lane (-) COS7 (monkey) cells.
Right lane (+) COS7 cells transfected with HA-CDYL-FLAG plasmid.
