Anti-Piwi antibody

• 产品名称

  Anti-Piwi抗体

• 描述

  兔多克隆抗体to Piwi

• 宿主

  Rabbit

• 经测试应用

  适用于: WBmore details

• 种属反应性

  与反应: Drosophila melanogaster
  

• 免疫原

  Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• This antibody gave a positive signal in Drosophila embryo nuclear extract

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: 98.98% PBS, 1% BSA
  
  Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.

• 浓度

• 批次浓度范围 100 µg 浓度为 0.5 - 0.9 mg/ml

• 纯度

  Immunogen affinity purified

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab5207于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

WB: 1/500. Detects a band of approximately 90 kDa (predicted molecular weight: 97 kDa). Can be blocked with Piwi peptide (ab14803).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

• 数据库链接

• Entrez Gene: 34521 Drosophila melanogaster

• SwissProt: Q9VKM1 Drosophila melanogaster

• 别名

• P-element induced wimpy testis antibody

• Piwi antibody

• 

  Western blot - Anti-Piwi antibody (ab5207)

  Anti-Piwi antibody (ab5207) at 1 µg/ml + Drosophila embryo nuclear extract (from melanogaster embryos 0-12Hr) at 20 µg
  
  Secondary
  IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/3000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size: 97 kDa
  Observed band size: 90 kDawhy is the actual band size different from the predicted?
  Additional bands at: 53 kDa. We are unsure as to the identity of these extra bands.
  
  

• 

  Western blot - Anti-Piwi antibody (ab5207)This image is courtesy of Ahmet Denli

  Drosophila embryo lysate, 8% TRIS SDS-PAGE.
  The arrow indicates Piwi at a predicted MW of 90 kDa.
  Pre-immune sera was used as a control and showed no equivalent band (data not shown).

  Drosophila embryo lysate, 8% TRIS SDS-PAGE. The arrow indicates Piwi at a predicted MW of 90 kDa. Pre-immune sera was used as a control and showed no equivalent band (data not shown). Ahmet Denli