Anti-SA2 antibody

• 产品名称

  Anti-SA2抗体
  参阅全部 SA2 一抗

• 描述

  山羊多克隆抗体to SA2

• 宿主

  Goat

• 特异性

  We have data to indicate that this antibody may not cross react with Xenopus laevis. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

• 经测试应用

  适用于: IP, WB, ICCmore details

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Synthetic peptide within Human SA2 aa 1100-1200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_006594.3 (GeneID 10735).
  Database link: Q8N3U4

• 阳性对照

• WB: HeLa nuclear cell extract. HeLa, HEK-293T and NIH/3T3 whole cell lysate. ICC: HeLa cells. IP: SA2 IP in HeLa whole cell lysate.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  pH: 7
  Preservative: 0.1% Sodium azide
  Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris

• 浓度

• 50 µg 浓度为 1 mg/ml

• 纯度

  Immunogen affinity purified

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab4463于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 10735 Human

• Entrez Gene: 20843 Mouse

• Omim: 300826 Human

• SwissProt: Q8N3U4 Human

• SwissProt: O35638 Mouse

• Unigene: 496710 Human

• Unigene: 624663 Human

• Unigene: 290422 Mouse

• 别名

• DKFZp686P168 antibody

• DKFZp781H1753 antibody

• FLJ25871 antibody

• SA 2 antibody

• SA-2 antibody

• SA2 antibody

• SCC3 homolog 2 antibody

• SCC3B antibody

• STAG 2 antibody

• stag2 antibody

• STAG2_HUMAN antibody

• Stromal antigen 2 antibody

• bA517O1.1 antibody

• Cohesin Subunit SA 2 antibody

• Cohesin subunit SA-2 antibody

• 

  Western blot - Anti-SA2 antibody (ab4463)

  Whole cell lysate (50 µg) from HeLa, HEK-293T, and NIH/3T3 cells prepared using NETN lysis buffer.

  ab4463 used for WB at 0.1 µg/ml.

• 

  Immunoprecipitation - Anti-SA2 antibody (ab4463)

  Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.

  Lane 1: ab4463 used for IP at 6 µg per reaction.

  Lane 2: Control IgG.

  For blotting immunoprecipitated SA2, ab4463 was used at 1 µg/ml.

• 

  Western blot - Anti-SA2 antibody (ab4463)

  Nuclear Extract from HeLa cells (~5 x 105 cells).

  ab4463 at 0.5 µg/ml.

  Detection: ECL with 15 second exposure.

  Nuclear Extract from HeLa cells (~5 x 10e5 cells).
  
  ab4463 at 0.5 µg/ml.
  
  Detection: ECL with 15 second exposure.

• 

  Immunocytochemistry - Anti-SA2 antibody (ab4463)

  Immunocytochemistry analysis of HeLa cells (extracted for 5 minutes at 4°C in 0.5% Triton in CSK buffer) labelling SA2 with ab4463 at 1µg/ml.