Anti-Transglutaminase 2 antibody [CUB 7402]

• 产品名称

  Anti-Transglutaminase 2抗体[CUB 7402]
  参阅全部 Transglutaminase 2 一抗

• 描述

  小鼠单克隆抗体[CUB 7402] to Transglutaminase 2

• 宿主

  Mouse

• 经测试应用

  适用于: IHC-P, WB, ICC/IF, Flow Cytmore details

• 种属反应性

  与反应: Mouse, Rabbit, Guinea pig, Cat, Dog, Human
  预测可用于: Rat

• 免疫原

  Full length native protein (purified) corresponding to Transglutaminase 2. Purified from liver.
  Database link: P08587

• 表位

  Epitope mapping for this antibody is amino acid 447-478

• 阳性对照

• IHC-P: Human breast carcinoma tissue. WB: A549 whole cell lysate; HeLa whole cell lysate; Huvec whole cell lysate

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.3
  Preservative: 0.05% Sodium azide
  Constituent: 1% BSA

• 纯度

  IgG fraction

• 克隆

  单克隆

• 克隆编号

  CUB 7402

• 骨髓瘤

  unknown

• 同种型

  IgG1

• 轻链类型

  unknown

The Abpromise guarantee

Abpromise™承诺保证使用ab2386于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 7052 Human

• Entrez Gene: 21817 Mouse

• Entrez Gene: 100352148 Rabbit

• Entrez Gene: 56083 Rat

• Omim: 190196 Human

• SwissProt: P21980 Human

• SwissProt: P21981 Mouse

• Unigene: 517033 Human

• Unigene: 330731 Mouse

别名

• ALPHA SUBUNIT antibody

• C polypeptide antibody

• EC 2.3.2.13 antibody

• epididymis secretory protein Li 45 antibody

• G alpha h antibody

• G[a]h antibody

• Gh CLASS G ALPHA h antibody

• GNAH antibody

• GNAH G PROTEIN antibody

• H POLYPEPTIDE antibody

• HEL-S-45 antibody

• Protein glutamine gamma glutamyltransferase 2 antibody

• Protein-glutamine gamma-glutamyltransferase 2 antibody

• TG 2 antibody

• TG(C) antibody

• TG2 antibody

• TGase C antibody

• TGase H antibody

• TGase-2 antibody

• TgaseII antibody

• TGC antibody

• TGC GUANINE NUCLEOTIDE BINDING PROTEIN antibody

• TGM2 antibody

• TGM2_HUMAN antibody

• Tissue transglutaminase antibody

• Transglutaminase 2 antibody

• Transglutaminase 2 C polypeptide antibody

• Transglutaminase C antibody

• Transglutaminase H antibody

• Transglutaminase-2 antibody

• tTG antibody

• tTGas antibody

• 

  Western blot - Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386)

  All lanes : Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386) at 1 µg/ml
  
  Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate
  Lane 2 : TGM2 knockout A549 (Human lung carcinoma cell line) whole cell lysate
  Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
  Lane 4 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Predicted band size: 77 kDa
  Observed band size: 77 kDa
  
  
  
  

  Lanes 1 - 4: Merged signal (red and green). Green - ab2386 observed at 77 kDa. Red - loading control, ab181602, observed at 37 kDa.

  ab2386 was shown to recognize  in wild-type A549 cells as signal was lost at the expected MW in TGM2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TGM2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab2386 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386)

  Immunohistochemical analysis of human breast carcinoma tissue labeling Transglutaminase 2 with ab2386. Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50) for 30 minutes at 22°C.

• 

  Flow Cytometry - Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386)

  Overlay histogram showing HCT116 cells stained with ab2386 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2386, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HCT116 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.