Anti-Cyclophilin A抗体
参阅全部 Cyclophilin A 一抗
兔多克隆抗体to Cyclophilin A
Rabbit
Detects recombinant human Cyclophilin A (CyPA), but does not detect endogenous levels of CyPA.
适用于: ICC/IF, WBmore details
与反应: Mouse, Human
预测可用于: Cow, Cat, Pig, Chimpanzee, Rhesus monkey, African green monkey
Recombinant full length protein corresponding to Human Cyclophilin A. Purified recombinant Human Cyclophilin A.
WB: CyPA; A549, U-87 MG, Jurkat cells; NIH/3T3, HeLa, mouse lung, colon and stomach tissue; HepG2, K562. ICC/IF: A431, HeLa cells
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
浓度
400 µl 浓度为 1 mg/ml
IgG fraction
多克隆
IgG
Abpromise™承诺保证使用ab3563于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| WB | (2) | 1/300. |
Entrez Gene: 5478 Human
Entrez Gene: 268373 Mouse
Omim: 123840 Human
SwissProt: P62937 Human
SwissProt: P17742 Mouse
Unigene: 356331 Human
Unigene: 598115 Human
Unigene: 5246 Mouse
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Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin A antibody (ab3563)
Immunocytochemistry analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized A431 cells staining Cyclophilin A with ab3563 at 1/250 dilution and DyLight-conjugated secondary antibody (green). F-actin was stained with red Rhodamine Phalloidin, and the nuclear counterstain was SlowFade® Gold Antifade Mountant with DAPI (blue). Negative control (e) is where no primary antibody was used.
Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin A antibody (ab3563)
Immunocytochemistry analysis of formalin-fixed 0.1% Triton X-100 permeabilized HeLa cells staining Cyclophilin A with ab3563 at 1/200 dilution and DyLight-conjugated secondary antibody (green). F-actin was stained with red phalloidin, and the nuclear counterstain was DAPI (blue).
Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin A antibody (ab3563)
Immunocytochemistry analysis of formalin-fixed 0.1% Triton X-100 permeabilized A431 cells staining Cyclophilin A with ab3563 at 1/200 dilution and DyLight-conjugated secondary antibody (green). F-actin was stained with red phalloidin, and the nuclear counterstain was DAPI (blue).
Western blot - Anti-Cyclophilin A antibody (ab3563)
All lanes : Anti-Cyclophilin A antibody (ab3563) at 1/100 dilution
Lane 1 : NIH/3T3 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : mouse lung tissue lysate
Lane 4 : mouse colon tissue lysate
Lane 5 : mouse stomach tissue lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjµgate at 1/2500 dilution
Developed using the ECL technique.
Western blot - Anti-Cyclophilin A antibody (ab3563)
All lanes : Anti-Cyclophilin A antibody (ab3563) at 1/3000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell extract
Lane 2 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, at 1/4000 dilution
Developed using the ECL technique.
Western blot - Anti-Cyclophilin A antibody (ab3563)
ab3563 at 1/300 dilution detecting 0.5ug recombinant human cyclophilin A by Western Blot (ECL).
Western blot - Anti-Cyclophilin A antibody (ab3563)
All lanes : Anti-Cyclophilin A antibody (ab3563) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : NIH-3T3
Lysates/proteins at 25 µg per lane.
Observed band size: 18 kDawhy is the actual band size different from the predicted?
