Anti-PPAR alpha (phospho S12)抗体
参阅全部 PPAR alpha 一抗
兔多克隆抗体to PPAR alpha (phospho S12)
Rabbit
The antibody is expected to bind both phospho and non phospho forms.
适用于: WB, ICC/IFmore details
与反应: Mouse, Human
预测可用于: Guinea pig, Dog
Synthetic peptide corresponding to Mouse PPAR alpha aa 1-100 (phospho S12).
Database link: P23204
WB: human U-87, MCF7, MDA-MB-231, C2C12, HepG2, and mouse NIH-3T3 ICC/IF: C2C12, 3T3-L1, U-87 MG cells
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab3484于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) | 1/100 - 1/1000. Predicted molecular weight: 52 kDa. |
| ICC/IF | (1) | 1/100 - 1/500. |
Entrez Gene: 100135604 Guinea pig
Entrez Gene: 5465 Human
Entrez Gene: 19013 Mouse
Omim: 170998 Human
SwissProt: O35507 Guinea pig
SwissProt: Q07869 Human
SwissProt: P23204 Mouse
Unigene: 103110 Human
Unigene: 710044 Human
Unigene: 212789 Mouse
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Western blot - Anti-PPAR alpha (phospho S12) antibody (ab3484)
All lanes : Anti-PPAR alpha (phospho S12) antibody (ab3484) at 1/200 dilution
Lane 1 : C2C12 cell lysate
Lane 2 : NIH-3T3 cell lysate
Lane 3 : 3T3-L1 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 52 kDa
Observed band size: 52 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PPAR alpha (phospho S12) antibody (ab3484)
Immunofluorescent analysis of Phospho-PPAR alpha pSer12 (green) showing staining in the nucleus of C2C12 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Phospho-PPAR alpha pSer12 polyclonal antibody (ab3484) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Immunocytochemistry/ Immunofluorescence - Anti-PPAR alpha (phospho S12) antibody (ab3484)
Immunofluorescent analysis of Phospho-PPAR alpha pSer12 (green) showing staining in the nucleus of 3T3-L1 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Phospho-PPAR alpha pSer12 polyclonal antibody (ab3484) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Western blot - Anti-PPAR alpha (phospho S12) antibody (ab3484)
All lanes : Anti-PPAR alpha (phospho S12) antibody (ab3484) at 1/1000 dilution
Lane 1 : U-87 MG with Skimmed milk
Lane 2 : MCF7 with Skimmed milk
Lane 3 : MDA-MB-231 with Skimmed milk
Lane 4 : C2C12 with Skimmed milk
Lane 5 : Hep G2 with Skimmed milk
Lane 6 : NIH/3T3 with Skimmed milk
Lysates/proteins at 20 µg per lane.
Blocking peptides at 5 % per lane.
Secondary
All lanes : Goat anti-rabbit IgG (H+L) at 1/2500 dilution
Predicted band size: 52 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PPAR alpha (phospho S12) antibody (ab3484)
Immunofluorescent analysis of Phospho-PPAR alpha pSer12 (green) showing staining in the nucleus of U-87 MG cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Phospho-PPAR alpha pSer12 polyclonal antibody (ab3484) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Immunocytochemistry/ Immunofluorescence - Anti-PPAR alpha (phospho S12) antibody (ab3484)This image is courtesy of an anonymous Abreview
ab3484 staining PPAR alpha (phospho S12) in Mouse neuronal cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 20 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS) for 18 hours at 4°C. A Cy2®-conjugated Donkey anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.
