Anti-Vitamin D Receptor抗体
参阅全部 Vitamin D Receptor 一抗
兔多克隆抗体to Vitamin D Receptor
Rabbit
适用于: IHC-Fr, ChIP, WB, IP, IHC-P, ChIP-sequencing, ICC/IFmore details
与反应: Mouse, Rat, Chicken, Human
预测可用于: Cow, Pig, Zebrafish, Saguinus oedipus
Synthetic peptide corresponding to Human Vitamin D Receptor aa 350-450.
Database link: P11473
IHC: Human jejunum
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Whole antiserum
多克隆
IgG
Abpromise™承诺保证使用ab3508于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| EMSA | Use at an assay dependent concentration. | |
| Gel Shift Assay | Use at an assay dependent concentration. | |
| IHC-Fr | (1) | Use at an assay dependent concentration. |
| ChIP | Use at an assay dependent concentration. PubMed: 17244627 | |
| WB | (3) | Use at an assay dependent concentration. Predicted molecular weight: 48 kDa. 1/100. Detects a band of approximately 53 kDa in COS-7 cells transfected with the human gene (predicted molecular weight: 48 kDa). This antibody supershifts DNA fragments that contain VDR response elements (e.g., rat osteocalcin and mouse osteopontin upstream elements). Read More |
| IP | Use at an assay dependent concentration. | |
| IHC-P | (1) | 1/2000 - 1/4000. |
| ChIP-sequencing | Use at an assay dependent concentration. PubMed: 21846776 | |
| ICC/IF | (1) | Use at an assay dependent concentration. |
Entrez Gene: 7421 Human
Entrez Gene: 22337 Mouse
Omim: 601769 Human
SwissProt: P11473 Human
SwissProt: P48281 Mouse
Unigene: 524368 Human
Unigene: 245084 Mouse
Unigene: 10911 Rat
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Receptor antibody (ab3508)
Ab3508 staining Human normal jejunum. Staining is localized to the nucleus.
Left panel: with primary antibody at 1/2000. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer, citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
