Anti-EBP50/NHERF-1抗体
参阅全部 EBP50/NHERF-1 一抗
兔多克隆抗体to EBP50/NHERF-1
Rabbit
Detects EBP50/NHERF-1 from human T84 and Calu3 cells as well as recombinant human protein.
适用于: WB, IP, IHC-Fr, ICC, IHC-Pmore details
与反应: Mouse, Rat, Dog, Human
预测可用于: Rabbit, Cow, Cynomolgus monkey
Recombinant fragment (His-tag) corresponding to Human EBP50/NHERF-1. All His-tag specific antibody has been removed.
Sequence:
RSASSDTSEELNSQDSP
WB: MCF7, T47D, SK-BR-3, HeLa, HepG2; Caco-2. IHC: mouse airway ICC: T47D cells
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
浓度
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab3452于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (4) | Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). |
| IP | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. PubMed: 15703401 | |
| ICC | Use a concentration of 2 µg/ml. | |
| IHC-P | Use a concentration of 10 µg/ml. |
Entrez Gene: 9368 Human
Entrez Gene: 26941 Mouse
Entrez Gene: 100009196 Rabbit
Omim: 604990 Human
SwissProt: O14745 Human
SwissProt: P70441 Mouse
SwissProt: Q28619 Rabbit
Unigene: 724482 Human
Unigene: 728760 Human
Unigene: 27842 Mouse
Unigene: 35142 Rat
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Solute carrier family 9 member 3 regulator 1 antibody
Immunocytochemistry - Anti-EBP50/NHERF-1 antibody (ab3452)
Immunocytochemistry analysis of 4% paraformaldehyde-fixed 0.1% Triton™ X-100 permeabilized T-47D cells staining EBP50/NHERF-1 with ab3452 at 2µg/ml, and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution (green). Nuclear counterstain was SlowFade® Gold Antifade Mountant with DAPI (blue), and F-actin was stained with Alexa Fluor® 555 Rhodamine Phalloidin (red). Negative control used no primary antibody.
Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)
All lanes : Anti-EBP50/NHERF-1 antibody (ab3452) at 0.2 µg/ml
Lane 1 : Membrane enriched extracts of MCF7 (human breast adenocarcinoma cell line)
Lane 2 : Membrane enriched extracts of T-47D (Human ductal breast epithelial tumor cell line)
Lane 3 : Membrane enriched extracts of SK-BR-3 (Human mammary gland adenocarcinoma cell line)
Lane 4 : Membrane enriched extracts of MDA-MB-231 (human breast adenocarcinoma cell line)
Lane 5 : Membrane enriched extracts of HeLa (human epithelial cell line from cervix adenocarcinoma)
Lane 6 : Membrane enriched extracts of HepG2 (human liver hepatocellular carcinoma cell line)
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution
Developed using the ECL technique.
Predicted band size: 50 kDa
Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)
All lanes : Anti-EBP50/NHERF-1 antibody (ab3452) at 1 µg/ml
Lane 1 : T-47D (Human ductal breast epithelial tumor cell line) whole cell extract
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell extract
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lane 4 : Caco-2 (Human colorectal adenocarcinoma cell line) whole cell extract
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/2500 dilution
Predicted band size: 50 kDa
Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)This image is courtesy of an anonymous abreview.
All lanes : Anti-EBP50/NHERF-1 antibody (ab3452) at 1/1000 dilution
All lanes : Whole cell lysate prepared from human HepG2 cells
Secondary
All lanes : HRP-conjugated goat polyclonal to rabbit IgG at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Exposure time: 40 seconds
Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)This image is courtesy of an anonymous abreview.
All lanes : Anti-EBP50/NHERF-1 antibody (ab3452) at 1/1000 dilution
All lanes : Whole tissue lysate prepared from mouse liver
Secondary
All lanes : HRP-conjugated goat polyclonal to rabbit IgG at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Exposure time: 30 seconds
Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)
Western blot detection of recombinant Human EBP50/NHERF-1 using ab3452.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBP50/NHERF-1 antibody (ab3452)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse airway tissue sections labeling EBP50/NHERF-1 with ab3452.
