Anti-Adiponectin antibody

• 产品名称

  Anti-Adiponectin抗体
  参阅全部 Adiponectin 一抗

• 描述

  兔多克隆抗体to Adiponectin

• 宿主

  Rabbit

• 特异性

  Detects Acrp30 from mouse serum. We have data to indicate that this antibody may not cross react with Human. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

• 经测试应用

  适用于: ICC, WBmore details

• 种属反应性

  与反应: Mouse
  

• 免疫原

  This product was produced with the following immunogens:
  Synthetic peptide corresponding to Mouse Adiponectin aa 1-100.
  Database link: Q60994
  
  Synthetic peptide corresponding to Mouse Adiponectin aa 150-250.
  Database link: Q60994

  Run BLAST with Run BLAST with 

  Run BLAST with Run BLAST with 

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituents: 0.1% BSA, 99% PBS

• 纯度

  Protein A purified

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab3455于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 11450 Mouse

• SwissProt: Q60994 Mouse

• Unigene: 3969 Mouse

• 别名

• Acrp 30 antibody

• ACRP30 antibody

• ADIPO_HUMAN antibody

• Adipocyte antibody

• Adipocyte C1q and collagen domain containing protein antibody

• Adipocyte complement related 30 kDa protein antibody

• Adipocyte complement related protein of 30 kDa antibody

• Adipocyte complement-related 30 kDa protein antibody

• adipocyte-specific secretory protein antibody

• Adiponectin antibody

• Adiponectin precursor antibody

• adiponectin, C1Q and collagen domain containing antibody

• Adipoq antibody

• Adipose most abundant gene transcript 1 antibody

• Adipose most abundant gene transcript 1 protein antibody

• Adipose specific collagen like factor antibody

• ADIPQTL1 antibody

• ADPN antibody

• APM 1 antibody

• apM-1 antibody

• APM1 antibody

• C1q and collagen domain-containing protein antibody

• GBP 28 antibody

• GBP28 antibody

• Gelatin binding protein antibody

• Gelatin binding protein 28 antibody

• gelatin-binding protein 28 antibody

• Gelatin-binding protein antibody

• OTTHUMP00000210047 antibody

• 30 kDa adipocyte complement related protein antibody

• 30 kDa adipocyte complement-related protein antibody

• ACDC antibody

• 

  Western blot - Anti-Adiponectin antibody (ab3455)

  All lanes : Anti-Adiponectin antibody (ab3455) at 2 µg/ml
  
  Lane 1 : Mouse adipose tissue extract in 3T3-L1 conditioned media
  Lane 2 : Mouse adipose tissue extract in NIH/3T3 conditioned media
  Lane 3 : Mouse adipose tissue extract in BM-MSC differentiated conditioned media
  
  Lysates/proteins at 30 µg per lane.
  
  Secondary
  All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/4000 dilution
  
  Developed using the ECL technique.
  

• 

  Immunocytochemistry - Anti-Adiponectin antibody (ab3455)

  Immunofluorescence analysis of Adiponectin was performed using 70% confluent 3T3-L1 cells differentiated with Adipogenesis Assay Kit (Cell-Based)(ab133102) for 5 days. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton TM X-100 for 10 min, blocked with 1% BSA for 1 hour at room temperature. 

  The cells were labeled with Adiponectin Polyclonal Antibody (ab3455) at 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then lebeled with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at a dilution of 1/2000 for 45 minutes at room temperature, panel A (green). Panel B (blue): nuclei were stained with DAPI. Panel C (red), F-actin was stained with Alexa Fluor®555 rhodamine phalloidin (ab235138) at 1/3000 dilution. Panel D represents the merged image showing cytoplasmic localization. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. 

• 

  Western blot - Anti-Adiponectin antibody (ab3455)

  Western blot detection of Adiponectin on mouse serum using ab3455.