Anti-Nicastrin antibody

• 产品名称

  Anti-Nicastrin抗体
  参阅全部 Nicastrin 一抗

• 描述

  兔多克隆抗体to Nicastrin

• 宿主

  Rabbit

• 经测试应用

  适用于: WBmore details

• 种属反应性

  与反应: Mouse, Rat, Human
  

• 免疫原

  Synthetic peptide corresponding to Human Nicastrin aa 650-750.

  Run BLAST with  Run BLAST with 

• 阳性对照

• WB: T98G, RSC96, mouse neuronal cells, HeLa, IMR-32, NIH/3T3, L6, mouse brain, rat brain

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituents: 0.1% BSA, 99% PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Immunogen affinity purified

• Primary antibody说明

  Amyloid beta peptide is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimer’s disease. This peptide is generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease termed beta-secretase (BACE). This enzyme is synthesized as a propeptide that must be modified to the mature and active form by the prohormone convertase, furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP and a membrane-bound remnant. This remnant is then processed by another protease termed gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide. Gamma-secretase is believed to be a multi-subunit complex containing presenilin-1 and 2 as central components. Found associated with the presenilins is the transmembrane glycoprotein nicastrin. Nicastrin has been found to bind to the carboxyl-terminus of beta APP and helps to modulate the production of the amyloid beta peptide. Also found in the neurofibrillary lesions associated with Alzheimer’s disease is a protein termed Tau. Tau is a neuronal microtubule-associated protein found predominantly on axons. Tau functions to promote tubulin polymerization and stabilize microtubules. Tau, in its hyperphosphorylated form, is the major constituent of paired helical filaments (PHF), which are the building block of neurofibrillary lesions found in brain tissue of Alzheimer’s diseased patients.

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab3444于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 23385 Human

• Entrez Gene: 59287 Mouse

• Entrez Gene: 289231 Rat

• Omim: 605254 Human

• SwissProt: Q92542 Human

• SwissProt: P57716 Mouse

• SwissProt: Q8CGU6 Rat

• Unigene: 517249 Human

• Unigene: 218203 Mouse

• Unigene: 51975 Rat

别名

• Anterior pharynx defective 2 antibody

• APH 2 antibody

• APH2 antibody

• ATAG1874 antibody

• KIAA0253 antibody

• Ncstn antibody

• NCT antibody

• NICA_HUMAN antibody

• Nicastrin antibody

• RP11 517F10.1 antibody

• RP11517F101 antibody

• 

  Western blot - Anti-Nicastrin antibody (ab3444)

  All lanes : Anti-Nicastrin antibody (ab3444) at 1 µg/ml
  
  Lane 1 : HeLa whole cell extract
  Lane 2 : IMR-32 whole cell extract (human neuroblastoma cell line)
  Lane 3 : NIH/3T3 whole cell extract (mouse embryo fibroblast cell line)
  Lane 4 : L6 whole cell extract (Rat skeletal muscle cell line)
  Lane 5 : mouse brain
  Lane 6 : rat brain
  
  Lysates/proteins at 60 µg per lane.
  
  Secondary
  All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution
  
  Developed using the ECL technique.
  
  Predicted band size: 78 kDa
  
  

• 

  Western blot - Anti-Nicastrin antibody (ab3444)

  All lanes : Anti-Nicastrin antibody (ab3444) at 2 µg/ml
  
  Lane 1 : T98G membrane extract (Human glioblastoma cell line)
  Lane 2 : RSC96 membrane extract (spontaneously immortalized rat Schwann cell line )
  
  Lysates/proteins at 30 µg per lane.
  
  Secondary
  All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/4000 dilution
  
  Developed using the ECL technique.
  
  Predicted band size: 78 kDa
  
  

• 

  Western blot - Anti-Nicastrin antibody (ab3444)

  Western blot detection of Nicastrin on mouse primary cultured neuronal cells using ab3444.