Anti-PICK1抗体
参阅全部 PICK1 一抗
兔多克隆抗体to PICK1
Rabbit
Detects protein interacting with Protein Kinase C (PICK 1). By Western blot, this antibody detects an ~52 kDa protein representing PICK 1 from AtT20 cell extract. Immunocytochemical staining of CV1 cells transfected with PICK 1 results in a diffuse staining pattern when expressed alone and coclustered when coexpressed with mGluR7.
适用于: WB, ICC/IFmore details
与反应: Mouse, Rat, Human
预测可用于: Cow, Cynomolgus monkey
Synthetic peptide corresponding to Mouse PICK1 aa 1-100.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 0.1% BSA, 99% PBS
浓度
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab3420于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) | Use a concentration of 2 µg/ml. Detects a band of approximately 52 kDa. |
| ICC/IF | (3) | Use at an assay dependent concentration. |
Entrez Gene: 9463 Human
Entrez Gene: 18693 Mouse
Omim: 605926 Human
SwissProt: Q9NRD5 Human
SwissProt: Q62083 Mouse
Unigene: 180871 Human
Unigene: 259464 Mouse
Unigene: 24750 Rat
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PICK 1 antibody
Pick1 antibody
PICK1_HUMAN antibody
PRKCA binding protein antibody
PRKCA-binding protein antibody
PRKCABP antibody
Protein interacting with C kinase 1 antibody
Protein interacting with PRKCA antibody
Protein interacting with PRKCA 1 antibody
Protein kinase C alpha binding protein antibody
Protein kinase C-alpha-binding protein antibody
Protein that interacts with C kinase 1 antibody
dJ1039K5 antibody
MGC15204 antibody
OTTHUMP00000028509 antibody
Western blot - Anti-PICK1 antibody (ab3420)This image is courtesy of an anonymous abreview.
Lane 1 : An myc antibody in PBST for 16 hours at 4°C
Lane 2 : Anti-PICK1 antibody (ab3420) at 1/500 dilution (in PBST for 16 hours at 4°C)
All lanes : Transfected CHO cells expressing human Pick1 tagged with myc
Lysates/proteins at 20 µg per lane.
Secondary
Lane 2 : An HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: ~52 kDawhy is the actual band size different from the predicted?
Additional bands at: ~40 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
Blocking Step: 5% Milk for 1 hour at 20°C
Immunocytochemistry/ Immunofluorescence - Anti-PICK1 antibody (ab3420)This image is courtesy of an anonymous abreview.
ab3420 staining PICK1 in mouse Hippocampal neurons by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in 0.1% saponin/PBS prior to blocking in 3% BSA for 1 hour at room temperature. The primary antibody was diluted 1/300 in 3% BSA, 0.1% saponin /PBS and incubated with the sample for 18 hours at 4 °C. The secondary antibody was Alexa Fluor® 455-conjugated Donkey anti-Rabbit polyclonal, diluted 1/1000.
Immunocytochemistry/ Immunofluorescence - Anti-PICK1 antibody (ab3420)This image is courtesy of an anonymous abreview.
ab3420 staining PICK1 in CHO cells stably transfected with Human PICK1 by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in 0.5% Triton X-100 prior to blocking in 10% serum for 1 hour at room temperature. The primary antibody was diluted 1/1000 in PBS, 0.5% Triton, 10% FCS and incubated with the sample for 2 hours at °C. The secondary antibody was Alexa Fluor® 488-conjugated Goat anti-Rabbit polyclonal, diluted 1/500.
Western blot - Anti-PICK1 antibody (ab3420)
Western blot of PICK1 on AtT20 cell lysate using ab3420.
