Anti-MSS1抗体
参阅全部 MSS1 一抗
兔多克隆抗体to MSS1
Rabbit
适用于: ICC/IF, WBmore details
与反应: Human
Recombinant full length protein corresponding to Human MSS1. Human recombinant proteasome 19S subunit S7.
WB: HeLa cell lysate. ICC/IF: SK-OV-3 cells.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 0.1% BSA, 99% PBS
IgG fraction
多克隆
IgG
Abpromise™承诺保证使用ab3322于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | 1/250. | |
| WB | 1/1000. By Western blot, this antibody detects a 47 kDa protein representing proteasome 19S subunit S7 from HeLa cell lysate. |
Entrez Gene: 5701 Human
Omim: 154365 Human
SwissProt: P35998 Human
Unigene: 437366 Human
mammalian suppressor of sgv-1 of yeast antibody
MSS1 antibody
Nbla10058 antibody
protease 26S subunit 7 antibody
Proteasome 19S S7 antibody
proteasome 26S ATPase subunit 2 antibody
Proteasome 26S subunit ATPase 2 antibody
Proteasome 26S subunit ATPase 2 antibody
Protein MSS1 antibody
PRS7_HUMAN antibody
PSMC2 antibody
putative protein product of Nbla10058 antibody
S7 antibody
26S protease complex antibody
26S protease regulatory subunit 7 antibody
26S proteasome AAA-ATPase subunit RPT1 antibody
Western blot - Anti-MSS1 antibody (ab3322)
Western blot of MSS1 on HeLa cell lysate using ab3322.
Immunocytochemistry/ Immunofluorescence - Anti-MSS1 antibody (ab3322)
Immunofluorescence analysis of MSS1 was performed using 70% confluent log phase SK-OV-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MSS1 Rabbit Polyclonal Antibody (ab3322) at 1/250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin (1/300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the control without primary antibody. The images were captured at 60X magnification.
