Anti-RanBP1 antibody

• 产品名称

  Anti-RanBP1抗体
  参阅全部 RanBP1 一抗

• 描述

  兔多克隆抗体to RanBP1

• 宿主

  Rabbit

• 经测试应用

  适用于: ICC/IF, WBmore details

• 种属反应性

  与反应: Mouse, Human
  预测可用于: Cow

• 免疫原

  Synthetic peptide corresponding to Human RanBP1 aa 1-18.
  Sequence:

  MAAAKDTHEDHDTSTENT

  
  (Peptide available as ab4938)

  Run BLAST with Run BLAST with 

• 阳性对照

• WB: HeLa, Human brain tissue and NIH-3T3 cell lysate. ICC/IF: HeLa cells.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituents: 0.1% BSA, 99% PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Immunogen affinity purified

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab2937于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 533251 Cow

• Entrez Gene: 5902 Human

• Entrez Gene: 19385 Mouse

• Omim: 601180 Human

• SwissProt: Q3T0M7 Cow

• SwissProt: P43487 Human

• SwissProt: P34022 Mouse

• Unigene: 24763 Human

• Unigene: 235287 Mouse

别名

• HTF9A antibody

• RAN binding protein 1 antibody

• Ran specific GTPase activating protein antibody

• Ran-binding protein 1 antibody

• Ran-specific GTPase-activating protein antibody

• RANBP1 antibody

• RANG_HUMAN antibody

• 

  Western blot - Anti-RanBP1 antibody (ab2937)

  All lanes : Anti-RanBP1 antibody (ab2937) at 1/1000 dilution
  
  Lane 1 : HeLa cell lysate
  Lane 2 : Human brain tissue lysate
  Lane 3 : NIH-3T3 cell lysate
  
  Lysates/proteins at 25 µg per lane.
  
  Secondary
  All lanes : HRP conjugated anti-rabbit
  
  Developed using the ECL technique.
  
  Predicted band size: 24 kDa
  Observed band size: 27 kDawhy is the actual band size different from the predicted?
  
  
  Exposure time: 1 minute
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-RanBP1 antibody (ab2937)

  Immunocytochemistry analysis of HeLa cells labeling RanB1 with ab2937 at 2 µg/mL. Cells were fixed in 4% Paraformaldehyde, permeabilized with 0.1% Triton™ X-100 for 10 minutes and blocked with 1% BSA for 1 hour at RT. Cells were incubated for 3 hours at RT and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1:2000 dilution for 45 minutes at RT (Panel A: green). Nuclei (Panel B: blue) were stained with DAPI. F-actin (Panel C: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1:300 dilution. Panel D is a merged image showing cytoplasmic localization. Panel E shows no primary antibody control. The images were captured at 60X magnification.