Anti-MDM2 antibody [SMP 14]

• 产品名称

  Anti-MDM2抗体[SMP 14]
  参阅全部 MDM2 一抗

• 描述

  小鼠单克隆抗体[SMP 14] to MDM2

• 宿主

  Mouse

• 经测试应用

  适用于: WBmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

• 表位

  Amino acids 154 - 167.

• 阳性对照

• HepG2 cell lysate (treated with 10uM Nutlin-3a for 24hrs)

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituent: PBS

• 浓度

• 10 µg 浓度为 1 mg/ml

• 100 µg 浓度为 1 mg/ml

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  SMP 14

• 骨髓瘤

  x63-Ag8.653

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab3110于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 4193 Human

• Omim: 164785 Human

• SwissProt: Q00987 Human

• Unigene: 484551 Human

• 别名

• Hdm 2 antibody

• Hdm2 antibody

• HDMX antibody

• MDM 2 antibody

• MDM2 antibody

• MDM2 oncogene E3 ubiquitin protein ligase antibody

• Mdm2 p53 E3 ubiquitin protein ligase homolog antibody

• Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa antibody

• MDM2_HUMAN antibody

• MDM2BP antibody

• Mouse Double Minute 2 antibody

• MTBP antibody

• Murine Double Minute Chromosome 2 antibody

• Oncoprotein Mdm2 antibody

• p53 Binding Protein Mdm2 antibody

• p53-binding protein Mdm2 antibody

• Ubiquitin protein ligase E3 Mdm2 antibody

• Ubiquitin protein ligase E3 Mdm2 antibody

• ACTFS antibody

• Double minute 2 protein antibody

• E3 ubiquitin-protein ligase Mdm2 antibody

• 

  Western blot - Anti-MDM2 antibody [SMP 14] (ab3110)

  All lanes : Anti-MDM2 antibody [SMP 14] (ab3110) at 2 µg/ml
  
  Lane 1 : HepG2 cell lysate
  Lane 2 : HepG2 treated with 10uM Nutlin-3a for 24hrs cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Performed under reducing conditions.
  
  Predicted band size: 55 kDa
  Observed band size: 75 kDawhy is the actual band size different from the predicted?
  
  
  
  

  Lanes 1 - 2: Merged signal (red and green). Green - ab3110 observed at 75 kDa. Red - loading control, ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37 kDa.

  ab3110 was shown to react with MDM2 in cell lysates subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab3110 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at 2 µg/ml and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.