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Anti-FOXG1 antibody

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产品详情
  • 产品名称

    Anti-FOXG1抗体
    参阅全部 FOXG1 一抗

  • 描述

    山羊多克隆抗体to FOXG1

  • 宿主

    Goat

  • 特异性

    This antibody will recognise both human FOXG1A and FOXG1B, which are almost completely identical.

  • 经测试应用

    适用于: Flow CytWBmore details

  • 种属反应性

    与反应: Human
    预测可用于: Rat

  • 免疫原

    Synthetic peptide corresponding to Human FOXG1 aa 457-469 (C terminal).
    Sequence:

    C-HQNQGSSSNPLIH


    Database link: P55316
    (Peptide available as ab22945)

    Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • WB: U251 cell lysate, Human cerebellum and cerebral cortex tissue lysates Flow Cyt: U251 cells

  • 常规说明


    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

  • 产品名称

    Anti-FOXG1抗体
    参阅全部 FOXG1 一抗

  • 描述

    山羊多克隆抗体to FOXG1

  • 宿主

    Goat

  • 特异性

    This antibody will recognise both human FOXG1A and FOXG1B, which are almost completely identical.

  • 经测试应用

    适用于: Flow CytWBmore details

  • 种属反应性

    与反应: Human
    预测可用于: Rat

  • 免疫原

    Synthetic peptide corresponding to Human FOXG1 aa 457-469 (C terminal).
    Sequence:

    C-HQNQGSSSNPLIH


    Database link: P55316
    (Peptide available as ab22945)

    Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • WB: U251 cell lysate, Human cerebellum and cerebral cortex tissue lysates Flow Cyt: U251 cells

  • 常规说明


    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • 存储溶液

    pH: 7.30
    Constituents: Tris buffered saline, 0.5% BSA

  • 浓度

    • 100 µg 浓度为 0.5 mg/m

  • 纯度

    Immunogen affinity purified

  • 纯化说明

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

  • 克隆

    多克隆

  • 同种型

    IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab3394于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用Ab评论说明
Flow Cyt

Use a concentration of 10 µg/ml.

WB

Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 54 kDa). 

A 1 hour primary incubation is recommended for this product. The additonal unknown band at 70kDa was successfully blocked with the immunising peptide.

Read More
  • 数据库链接

  • 别名

    • BF2 antibody

    • Brain factor 1 antibody

    • Brain factor 2 antibody

    • FHKL antibody

    • FKH2 antibody

    • FKHL1 antibody

    • FKHL2 antibody

    • FKHL3 antibody

    • FKHL4 antibody

    • Forkhead box protein G1 antibody

    • Forkhead box protein G1A antibody

    • Forkhead box protein G1B antibody

    • Forkhead box protein G1C antibody

    • Forkhead like 1 antibody

    • Forkhead like 2 antibody

    • Forkhead like 3 antibody

    • Forkhead like 4 antibody

    • Forkhead-related protein FKHL1 antibody

    • Forkhead-related protein FKHL2 antibody

    • Forkhead-related protein FKHL3 antibody

    • FOXG1 antibody

    • FOXG1_HUMAN antibody

    • FOXG1A antibody

    • FOXG1B antibody

    • FOXG1C antibody

    • HBF 1 antibody

    • HBF G2 antibody

    • hBF-2 antibody

    • HBF2 antibody

    • HFK1 antibody

    • HFK2 antibody

    • HFK3 antibody

    • KHL2 antibody

    • Oncogene QIN antibody

    • QIN antibody

    • BF-1 antibody

    • BF-2 antibody

    • BF1 antibody

  • Flow Cytometry - Anti-FOXG1 antibody (ab3394)

    Flow Cytometry - Anti-FOXG1 antibody (ab3394)

    Flow cytometric analysis of paraformaldehyde fixed U251 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

  • Western blot - Anti-FOXG1 antibody (ab3394)

    Western blot - Anti-FOXG1 antibody (ab3394)

    Anti-FOXG1 antibody (ab3394) at 1 µg/ml + U251 cell lysate at 35 µg

    Predicted band size: 54 kDa



    Detected by chemiluminescence.

  • Western blot - Anti-FOXG1 antibody (ab3394)

    Western blot - Anti-FOXG1 antibody (ab3394)

    All lanes : Anti-FOXG1 antibody (ab3394) at 0.5 µg/ml

    Lane 1 : Human Cerebellum tissue lysate
    Lane 2 : Cerebral Cortex tissue lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 54 kDa



    Detected by chemiluminescence.