Anti-beta COP抗体
参阅全部 beta COP 一抗
兔多克隆抗体to beta COP
Rabbit
适用于: IP, WB, ICC/IFmore details
与反应: Mouse, Rat, Hamster, Cow, Human, Non human primates
预测可用于: Chicken, Pig
Synthetic peptide corresponding to Rat beta COP aa 496-513.
Sequence:
EAGELKPEEEITVGPVQK
ICC/IF: L6 and NIH/3T3 cells; Rat brain homogenate. WB: HeLa, NIH/3T3, PC-3, C2C12, HCT116 and COLO 205 cell lysates; Mouse brain tissue lysate.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
浓度
100 µl 浓度为 4.4 mg/ml
Ammonium Sulphate Precipitation
Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COP I and COP II) and although they have mechanistic parallels, they are molecularly distinct. The COP I coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COP I) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COP I, and ARF. COP I has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COP II facilitates anterograde transport between these subcellular organelles. COP II has been shown to be independently and selectively recruited to the ER relative to COP I subunits.
多克隆
IgG
Abpromise™承诺保证使用ab2899于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | (1) | Use at an assay dependent concentration. PubMed: 18556652 |
| WB | (8) | 1/1000. Detects a band of approximately 110 kDa. |
| ICC/IF | (6) | 1/2000. Golgi localization of beta-COP is sensitive to treatment with brefeldin A or ATP depletion. |
Entrez Gene: 423063 Chicken
Entrez Gene: 1315 Human
Entrez Gene: 70349 Mouse
Omim: 600959 Human
SwissProt: Q5ZIA5 Chicken
SwissProt: P53618 Human
SwissProt: Q9JIF7 Mouse
Unigene: 339278 Human
Unigene: 277024 Mouse
Unigene: 4327 Rat
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Immunocytochemistry/ Immunofluorescence - Anti-beta COP antibody (ab2899)
Immunolocalization of beta COP in NIH-3T3 cells using ab2899
Western blot - Anti-beta COP antibody (ab2899)
All lanes : Anti-beta COP antibody (ab2899) at 2 µg/ml
Lane 1 : Mouse brain tissue lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 3 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
Lane 4 : C2C12 (Mouse myoblast cell line) whole cell lysate
Lane 5 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 6 : HCT116 (Human colorectal carcinoma cell line) whole cell lysate
Lane 7 : COLO 205 (Human colon adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Western blot analysis was performed on membrane enriched extracts (30 µg) of cells. The blots were probed with ab2899 (2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate. A ~107 kDa band corresponding beta COP was observed across tissue and cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE®4-12 % Bis-Tris gel, XCell SureLock™ Electrophoresis System and Novex® Sharp Pre-Stained Protein Standard. Resolved proteins were then transferred onto a nitrocellulose membrane by Pierce™ Power Blotter System. The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate.
Western blot - Anti-beta COP antibody (ab2899)
All lanes : Anti-beta COP antibody (ab2899) at 2 µg/ml
Lane 1 : Untransfected HeLa cell lysate
Lane 2 : HeLa (transfected with non-targeting scrambled siRNA) cell lysate
Lane 3 : HeLa (transfected with beta COP specific siRNAs) cell lysate
Knockdown of beta COP was achieved by transfecting HeLa with beta COP specific siRNAs. Western blot analysis was performed using Membrane Enriched extracts from the beta COP knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with ab2899 at 2ug/ml and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (1:4000 dilution).
Immunocytochemistry/ Immunofluorescence - Anti-beta COP antibody (ab2899)
Immunofluorescent analysis of beta COP was performed using 70% confluent log phase L6 (Rat skeletal muscle cell line) cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab2899 at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Western blot - Anti-beta COP antibody (ab2899)
Western blot of beta-COP on rat brain homogenate using ab2899. Western blot of beta-COP on rat brain homogenate using ab2899.
