Anti-STUB1/CHIP antibody

• 产品名称

  Anti-STUB1/CHIP抗体
  参阅全部 STUB1/CHIP 一抗

• 描述

  兔多克隆抗体to STUB1/CHIP

• 宿主

  Rabbit

• 特异性

  Detects human carboxyl terminus of hsc70-interacting protein (CHIP).Detects a band of approximately 35 kDa representing CHIP from COS-1 cells overexpressing the human gene including a non-specific band at 80kDa . A customer reported that in mouse cerebrum the antibody detects a band of 35kDa as well as two small mon-specific at 44 and 70 kDa.

• 经测试应用

  适用于: WBmore details

• 种属反应性

  与反应: Mouse, Human
  预测可用于: Chicken

• 免疫原

  Synthetic peptide corresponding to Human STUB1/CHIP aa 218-232.
  Sequence:

  VDEKRKKRDIPDYLC

  
  (Peptide available as ab4934)

  Run BLAST with Run BLAST with 

• 阳性对照

• WB: mouse brain, MCF-7 cells, transfected COS-1 cells

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituents: 0.1% BSA, 99% PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Immunogen affinity purified

• Primary antibody说明

  A recently identified protein, termed carboxyl terminus of hsc70-interacting protein (CHIP), has been shown to interact both with the constitutive form of hsc70 and the stress inducible form, hsp70. This novel 35 kDa cytoplasmic protein has been shown to be highly expressed in striated muscle in vivo. Additional studies have shown that this protein is expressed over a broad range of cultured tissues. Through immunoprecipitation experiments, CHIP has been shown to directly bind to the carboxyl terminus of hsc70 and hsp70 where it decreases ATPase activity and reduces overall chaperone efficiency. CHIP has also been identified as an important protein in the ubiquitin-proteasome system. CHIP contains a U-box domain and acts as an E3 ubiquitin-ligase in conjunction with hsc70 and hsp90.

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab2917于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 426918 Chicken

• Entrez Gene: 10273 Human

• Entrez Gene: 56424 Mouse

• Omim: 607207 Human

• SwissProt: Q5ZHY5 Chicken

• SwissProt: Q9UNE7 Human

• SwissProt: Q9WUD1 Mouse

• Unigene: 592081 Human

• 别名

• Carboxy terminus of Hsp70 interacting protein antibody

• Carboxy terminus of Hsp70-interacting protein antibody

• Carboxy terminus of Hsp70p interacting protein antibody

• CHIP antibody

• CHIP_HUMAN antibody

• CLL associated antigen KW 8 antibody

• CLL-associated antigen KW-8 antibody

• E3 ubiquitin protein ligase CHIP antibody

• E3 ubiquitin-protein ligase CHIP antibody

• Heat shock protein A binding protein 2 (c terminal) antibody

• HSPABP2 antibody

• NY CO 7 antibody

• PP1131 antibody

• SDCCAG7 antibody

• Serologically defined colon cancer antigen 7 antibody

• STIP1 homology and U Box containing protein 1 antibody

• STIP1 homology and U box containing protein 1 E3 ubiquitin protein ligase antibody

• STIP1 homology and U box-containing protein 1 antibody

• STUB 1 antibody

• STUB1 antibody

• UBOX 1 antibody

• UBOX1 antibody

• Antigen NY CO 7 antibody

• Antigen NY-CO-7 antibody

• C terminus of Hsp70-interacting protein antibody

• 

  Western blot - Anti-STUB1/CHIP antibody (ab2917)

  Western blot detection of transfected COS-1 cells expressing STUB1/CHIP using ab2917.

• 

  Western blot - Anti-STUB1/CHIP antibody (ab2917)

  Western blot analysis of STUB1/CHIP was performed by loading 20ug of total protein extracted from the left hemisphere (Left lane) or right hemisphere (right lane) of a normal C57BL/6 mouse brain per well on an SDS-PAGE gel. Proteins were transferred to a membrane, blocked with 5% non-fat dry milkand probed with a STUB1/CHIP polyclonal antibody (ab2915) at a dilution of 1:1000, followed by a HRP-conjugated goat anti-rabbit IgG secondary antibody. Detection was performed using a chemiluminescent substrate.

• 

  Western blot - Anti-STUB1/CHIP antibody (ab2917)