Anti-Calmodulin 1/2/3 antibody [2D1]

• 产品名称

  Anti-Calmodulin 1/2/3抗体[2D1]
  参阅全部 Calmodulin 1/2/3 一抗

• 描述

  小鼠单克隆抗体[2D1] to Calmodulin 1/2/3

• 宿主

  Mouse

• 特异性

  This antibody detects calmodulin. It does not detect parvalbumin, tropinin, S-100, or myosin light chain kinase (MLCK).By Western blot, this antibody detects a 17 kDa protein representing calmodulin from Dictyostelium cell lysate. Immunohistochemical staining of calmodulin in Dictyostelium cells with this antibody results in staining of the contractile vacuoles.

• 经测试应用

  适用于: WB, ELISA, ICC/IF, Flow Cyt, IP, IHC-Pmore details

• 种属反应性

  与反应: Mouse, Rat, Chicken, Cow, Human, Dictyostelium discoideum
  预测可用于: Wheat

• 免疫原

  Full length protein corresponding to Calmodulin 1/2/3. Calmodulin purified from Dictyostelium discoideum.

• 阳性对照

• ICC/IF: HeLa, A2058, C6 cells. IHC-P: Rat testis and cerebellum tissue. Flow Cyt: HeLa, C6, MCF-7 and PC-12 cells.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituent: 99% PBS

• 纯度

  Protein A purified

• Primary antibody说明

  Calmodulin is a small, highly conserved calcium binding protein found in all eukaryotic cells. With the capacity to bind up to four calcium ions, this 17 kDa protein acts as an important intracellular receptor for regulatory calcium signals. As it binds calcium, calmodulin undergoes conformational changes which can increase its affinity for target proteins. It acts both directly, through interaction with key target enzymes, and indirectly, via specific kinases. Studies have found that calmodulin participates in the regulation of several biological processes including energy and biosynthetic metabolism, cell motility, exocytosis, cytoskeletal assembly, and intracellular modulation of both cAMP and calcium concentrations.

• 克隆

  单克隆

• 克隆编号

  2D1

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab2860于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 416692 Chicken

• Entrez Gene: 326597 Cow

• Entrez Gene: 520277 Cow

• Entrez Gene: 808 Human

• Entrez Gene: 12315 Mouse

• Entrez Gene: 24244 Rat

• Omim: 114182 Human

• Omim: 114183 Human

• SwissProt: P62157 Cow

• SwissProt: 114180 Human

• SwissProt: P0DP23 Human

• SwissProt: P0DP24 Human

• SwissProt: P0DP25 Human

• SwissProt: P62158 Human

• SwissProt: Q9BJF5 Human

• SwissProt: P62204 Mouse

• SwissProt: P62161 Rat

• 形式

  There are three genes which encode an identical calcium binding protein which is one of the four subunits of phosphorylase kinase.

• 别名

• CALM antibody

• CALM1 antibody

• CALM2 antibody

• CALM3 antibody

• CALML2 antibody

• calmodulin 1 (phosphorylase kinase, delta) antibody

• Calmodulin 1 antibody

• Calmodulin 2 (phosphorylase kinase, delta) antibody

• Calmodulin 2 antibody

• Calmodulin 3 (phosphorylase kinase, delta) antibody

• Calmodulin 3 antibody

• CAM 2 antibody

• CAM 3 antibody

• CAM antibody

• CAM I antibody

• CAM1 antibody

• CAM2 antibody

• CAM3 antibody

• CAMB antibody

• CAMC antibody

• CAMI antibody

• CAMII antibody

• CAMIII antibody

• CPVT4 antibody

• DD132 antibody

• FLJ99410 antibody

• LP7057 protein antibody

• PHKD antibody

• PHKD2 antibody

• PHKD3 antibody

• phosphorylase kinase delta antibody

• phosphorylase kinase, delta subunit antibody

• CALM 1 antibody

• CALM 2 antibody

• CALM 3 antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Calmodulin 1/2/3 antibody [2D1] (ab2860)

  Immunofluorescent analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Calmodulin with ab2860. Calmodulin staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calmodulin ab2860 at a dilution of 1:50 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmodulin 1/2/3 antibody [2D1] (ab2860)

  Immunohistochemistry was performed on normal biopsies of deparaffinized rat testis tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Calmodulin ab2860 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  
  

• 

  Flow Cytometry - Anti-Calmodulin 1/2/3 antibody [2D1] (ab2860)

  Flow cytometry analysis of Calmodulin showing positive staining in the cytoplasm of C6 (Rat glial tumor cell line) cells compared to an isotype control (blue). Cells were harvested and adjusted to a concentration of 1-5x10^6 cells/ml. Cells were then fixed with 2% paraformaldehyde and washed with PBS. Cells were penetrated by dropping the supernatant and adding 90% methanol followed by incubation for 10 minutes at room temperature. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ab2860 at 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Calmodulin 1/2/3 antibody [2D1] (ab2860)

  Immunofluorescent analysis of C6 (Rat glial tumor cell line) cells labeling Calmodulin ab2860. Calmodulin staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calmodulin ab2860 at a dilution of 1:50 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmodulin 1/2/3 antibody [2D1] (ab2860)

  Immunohistochemistry was performed on normal biopsies of deparaffinized rat cerebellum tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Calmodulin ab2860 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  
  

• 

  Flow Cytometry - Anti-Calmodulin 1/2/3 antibody [2D1] (ab2860)

  Flow cytometry analysis of Calmodulin showing positive staining in the cytoplasm of PC-12 (Rat adrenal gland pheochromocytoma cell line) cells compared to an isotype control (blue). Cells were harvested and adjusted to a concentration of 1-5x10^6 cells/ml. Cells were then fixed with 2% paraformaldehyde and washed with PBS. Cells were penetrated by dropping the supernatant and adding 90% methanol followed by incubation for 10 minutes at room temperature. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ab2860 at 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Calmodulin 1/2/3 antibody [2D1] (ab2860)

  Immunofluorescent analysis of A2058 (Human metastatic melanoma cell line) cells labeling Calmodulin ab2860. Calmodulin staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calmodulin ab2860 at a dilution of 1:50 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
  
  

• 

  Flow Cytometry - Anti-Calmodulin 1/2/3 antibody [2D1] (ab2860)

  Flow cytometry analysis of Calmodulin showing positive staining in the cytoplasm of MCF7 (Human breast adenocarcinoma cell line) cells compared to an isotype control (blue). Cells were harvested and adjusted to a concentration of 1-5x10^6 cells/ml. Cells were then fixed with 2% paraformaldehyde and washed with PBS. Cells were penetrated by dropping the supernatant and adding 90% methanol followed by incubation for 10 minutes at room temperature. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ab2860 at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

• 

  Flow Cytometry - Anti-Calmodulin 1/2/3 antibody [2D1] (ab2860)

  Overlay histogram showing HeLa cells stained with ab2860 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2860, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmodulin 1/2/3 antibody [2D1] (ab2860)

  Immunolocalization of calmodulin in rat brain cells using ab2860 (1:100)