Anti-NCX1 antibody [C2C12]

• 产品名称

  Anti-NCX1抗体[C2C12]
  参阅全部 NCX1 一抗

• 描述

  小鼠单克隆抗体[C2C12] to NCX1

• 宿主

  Mouse

• 经测试应用

  适用于: Flow Cyt (Intra), IHC-P, IHC-Frmore details

• 种属反应性

  与反应: Human
  预测可用于: Mouse, Rat, Rabbit, Guinea pig, Dog, Pig

• 免疫原

  Full length native protein (purified) corresponding to Dog NCX1. Purified from canine cardiac sodium/calcium exchanger.

• 表位

  This antibody recognizes an epitope between amino acids 371-525 on the intracellular side of the plasma membrane.

• 阳性对照

• IHC-P: Normal human kidney tissue. IHC-Fr: Normal human kidney tissue

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 产品名称

  Anti-NCX1抗体[C2C12]
  参阅全部 NCX1 一抗

• 描述

  小鼠单克隆抗体[C2C12] to NCX1

• 宿主

  Mouse

• 经测试应用

  适用于: Flow Cyt (Intra), IHC-P, IHC-Frmore details

• 种属反应性

  与反应: Human
  预测可用于: Mouse, Rat, Rabbit, Guinea pig, Dog, Pig

• 免疫原

  Full length native protein (purified) corresponding to Dog NCX1. Purified from canine cardiac sodium/calcium exchanger.

• 表位

  This antibody recognizes an epitope between amino acids 371-525 on the intracellular side of the plasma membrane.

• 阳性对照

• IHC-P: Normal human kidney tissue. IHC-Fr: Normal human kidney tissue

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

The Abpromise guarantee

Abpromise™承诺保证使用ab2869于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 6546 Human

• Entrez Gene: 20541 Mouse

• Entrez Gene: 397365 Pig

• Entrez Gene: 100328570 Rabbit

• Entrez Gene: 29715 Rat

• Omim: 182305 Human

• SwissProt: P23685 Dog

• SwissProt: P48766 Guinea pig

• SwissProt: P32418 Human

• SwissProt: P70414 Mouse

• SwissProt: Q01728 Rat

• Unigene: 31961 Human

• Unigene: 468274 Human

• Unigene: 728911 Human

• Unigene: 265834 Mouse

• Unigene: 118972 Rat

别名

• CNC antibody

• DKFZp779F0871 antibody

• MGC119581 antibody

• Na(+)/Ca(2+)-exchange protein 1 antibody

• Na+/Ca2+ exchange protein 1 antibody

• Na+/Ca2+ exchanger antibody

• NAC1_HUMAN antibody

• NCX 1 antibody

• NCX antibody

• NCX1 antibody

• SLC8A1 antibody

• SLC8A1 protein antibody

• Sodium Calcium Exchanger antibody

• Sodium/calcium exchanger 1 antibody

• Solute carrier family 8 (sodium/calcium exchanger) member 1 antibody

• Solute carrier family 8 member 1 antibody

• 

  Immunohistochemistry (Frozen sections) - Anti-NCX1 antibody [C2C12] (ab2869)

  IHC image of NCX1 staining in a section of frozen normal human kidney*. The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature.  The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab2869 at 1µg/ml and ab6046 (Rabbit polyclonal to beta Tubulin - Loading Control) at 1/1000. The section was then incubated with ab150117 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488), 1/1000)) (shown in green) and ab150080 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594), 1/1000) (shown in red) for 1 hour at room temperature. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. The secondary-only control insert image is taken from an identical assay without primary antibody. The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times. *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCX1 antibody [C2C12] (ab2869)

  IHC image of NCX1 staining in a section of formalin-fixed paraffin-embedded normal human kidney* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab2869, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• 

  Flow Cytometry (Intracellular) - Anti-NCX1 antibody [C2C12] (ab2869)

  Overlay histogram showing HEK293 cells stained with ab2869 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2869, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCX1 antibody [C2C12] (ab2869)This image is courtesy of an anonymous Abreview

  ab2869 staining NCX1 in Human kidney tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.05% Tween20 and blocked with 5% normal goat serum in 1XPBS + 0.05% Tween20 for 1 hour at 25°C; antigen retrieval was by heat mediation in sodium citrate (pH 6.0) buffer. Samples were incubated with primary antibody (1/100 in blocking buffer) for 1 hour at 25°C. Ab47827 (1/500) was used as the secondary antibody.

  See Abreview