Anti-eIF4EBP1抗体
参阅全部 eIF4EBP1 一抗
兔多克隆抗体to eIF4EBP1
Rabbit
This antibody detects alpha (weakly), beta and gamma (strongly) isoforms of eIF-4EBP1.
适用于: IHC-P, WBmore details
与反应: Rat, Human
预测可用于: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide (Human) - which represents a portion of human eIF-4EBP1 encoded in part by exons 2and 3.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Affinity purified using the immunising peptideimmobilized on solid support.
多克隆
IgG
Abpromise™承诺保证使用ab2606于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) | 1/5000 - 1/20000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
| WB | (2) | Use at an assay dependent concentration. Predicted molecular weight: 13 kDa. |
Entrez Gene: 1978 Human
Omim: 602223 Human
SwissProt: Q13541 Human
Unigene: 411641 Human
Unigene: 11161 Rat
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Western blot - Anti-eIF4EBP1 antibody (ab2606)
Ab2606 at a 1:4000 dilution staining alpha, beta and gamma isoforms of eIF-4EBP1 in 100µg of RIN-m5F (Rat insulinoma cell line) whole cell lysate
after exposure to high (lane 1) and low (lane 2) concentrations of leucine. Ab2606 at a 1:4000 dilution staining alpha, beta and gamma isoforms of eIF-4EBP1 in 100µg of RINm5F cell lysate after exposure to high (lane 1) and low (lane 2) concentrations of leucine.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4EBP1 antibody (ab2606)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling eIF4EBP1 with ab2606 at 1/20000 (0.05µg/ml). Detection: DAB.
Western blot - Anti-eIF4EBP1 antibody (ab2606)
All lanes : Anti-eIF4EBP1 antibody (ab2606) at 1/1000 dilution
Lane 1 : Recombinant Human eIF4EBP1 protein (ab60007) at 0.01 µg
Lane 2 : Recombinant Human eIF4EBP1 protein (ab60007) at 0.001 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 13 kDa
Exposure time: 10 seconds
