Anti-Melanophilin抗体
山羊多克隆抗体to Melanophilin
Goat
适用于: WB, ICC/IFmore details
与反应: Mouse
预测可用于: Rat
Synthetic peptide corresponding to Mouse Melanophilin aa 550 to the C-terminus (C terminal).
Database link: Q91V27
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
浓度
100 µg 浓度为 0.5 mg/ml
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
多克隆
IgG
Abpromise™承诺保证使用ab2716于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 65 kDa. 1 hour primary incubation is recommended for this product. | |
| ICC/IF | Use a concentration of 0.5 µg/ml. |
Entrez Gene: 171531 Mouse
SwissProt: Q91V27 Mouse
Unigene: 105208 Mouse
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Western blot - Anti-Melanophilin antibody (ab2716)
All lanes : Anti-Melanophilin antibody (ab2716) at 0.5 µg/ml
Lane 1 : total melanocyte PNS (200ug per lane) of wild-type
Lane 2 : total melanocyte PNS (200ug per lane) of leaden (Mlph null) mice
Predicted band size: 65 kDa
Observed band size: 81 kDawhy is the actual band size different from the predicted?
ab2716 staining (0.5ug/ml) of total melanocyte PNS (200ug per lane) of wild-type and leaden (Mlph null) mice. The upper band may represent the stacking-separating gel interface.
Immunocytochemistry/ Immunofluorescence - Anti-Melanophilin antibody (ab2716)
Wild-type C57bl6 derived melanocytes were stained with ab2716 (0.5ug/ml) and detected with Alexa568 conjugated donkey anti-sheep second antibodies. Brightfield image shows melanosome distribution. Data kindly provided by A N Hume, Imperial College.
