Anti-HCN2抗体
参阅全部 HCN2 一抗
兔多克隆抗体to HCN2
Rabbit
适用于: WB, ICC/IFmore details
与反应: Mouse, Rat
预测可用于: Human
Synthetic peptide corresponding to Human HCN2 aa 874-889.
Sequence:
G-LDPQDSARSRLSSNL
Database link: Q9UL51
WB: rat brain lysate ICC/IF: Neuro-2a (Mouse neuroblastoma cell line)
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab19346于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/100. Detects a band of approximately 100,115 kDa. | |
| ICC/IF | Use a concentration of 2 µg/ml. |
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Unigene: 12956 Mouse
Unigene: 224995 Rat
Entrez Gene: 610 Human
Entrez Gene: 15166 Mouse
Omim: 602781 Human
SwissProt: Q9UL51 Human
SwissProt: O88703 Mouse
Unigene: 124161 Human
Brain cyclic nucleotide-gated channel 2 antibody
HAC1 antibody
Hcn2 antibody
HCN2_HUMAN antibody
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 antibody
BCNG-2 antibody
BCNG2 antibody
Brain cyclic nucleotide gated channel 2 antibody
Western blot - Anti-HCN2 antibody (ab19346)
ab19346 has been successfully used in Western blot procedures. By Western blot, this antibody detects bands at ~100 kDa and ~115 kDa representing the non-glycosylated and glycosylated form of HCN2 in rat brain extract.
Immunocytochemistry/ Immunofluorescence - Anti-HCN2 antibody (ab19346)
Immunofluorescent analysis of Neuro-2a (Mouse neuroblastoma cell line) cells on 70% confluent log phase labeling HCN2. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labelled with ab19346 at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) secondary antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (1/300 dilution). Panel d is a merged image showing localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
