Anti-Ubiquitin antibody

• 产品名称

  Anti-Ubiquitin抗体
  参阅全部 Ubiquitin 一抗

• 描述

  兔多克隆抗体to Ubiquitin

• 宿主

  Rabbit

• 特异性

  It can identify free ubiquitin as well as ubiquinated proteins. The antibody recognizes polyubiquitin chains more strongly than monoubiquitinated molecules.

• 经测试应用

  适用于: WBmore details

• 种属反应性

  与反应: Mouse, Human, Saccharomyces cerevisiae
  

• 免疫原

  Full length native protein (purified) corresponding to Cow Ubiquitin conjugated to Keyhole Limpet Haemocyanin (KLH).

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.20
  Preservative: 0.09% Sodium azide
  Constituents: PBS, 50% Glycerol

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab19247于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

  hide

• SwissProt: P62991 Mouse

• Unigene: 356190 Human

• Unigene: 520348 Human

• Unigene: 282093 Mouse

• Unigene: 371592 Mouse

• Entrez Gene: 7314 Human

• Entrez Gene: 22187 Mouse

• SwissProt: P0CG47 Human

• SwissProt: P0CG48 Human

• SwissProt: P62979 Human

• SwissProt: P62987 Human

• SwissProt: P62988 Human

• SwissProt: P0CG49 Mouse

• 别名

• MGC8385 antibody

• Polyubiquitin B antibody

• RPS 27A antibody

• RPS27A antibody

• UBA 52 antibody

• UBA 80 antibody

• UBA52 antibody

• UBA80 antibody

• UBB antibody

• UBB_HUMAN antibody

• UBC antibody

• UBCEP 1 antibody

• UBCEP 2 antibody

• UBCEP1 antibody

• UBCEP2 antibody

• Ubiquitin antibody

• Ubiquitin B antibody

• Epididymis secretory protein Li 50 antibody

• FLJ25987 antibody

• HEL S 50 antibody

• 

  Western blot - Anti-Ubiquitin antibody (ab19247)

  All lanes :
  
  Lane 1 : HeLa cell lysate (Control, untreated)
  Lane 2 : HeLa cells treated with MG132 (50 uM for 90 min)
  Lane 3 : Mouse Brain
  
  Lysates/proteins at 20 µg per lane.
  
  Performed under reducing conditions.
  
  Predicted band size: 10 kDa
  
  
  
  

  This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes.  ab19247 and ab8245 (loading control to GAPDH) were diluted 1/200  and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging using the Licor Odyssey CLx.

• 

  Western blot - Anti-Ubiquitin antibody (ab19247)

  Anti-Ubiquitin antibody (ab19247) at 1/1000 dilution + Heat-shocked HeLa cell lysate
  
  Predicted band size: 10 kDa