Anti-Cdc20抗体[BA8]
参阅全部 Cdc20 一抗
小鼠单克隆抗体[BA8] to Cdc20
Mouse
适用于: Flow Cytmore details
与反应: Human
Synthetic C terminal peptide (Xenopus laevis).
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS
浓度
100 µg 浓度为 0.5 mg/ml
Protein A/G purified
单克隆
BA8
IgG2a
Abpromise™承诺保证使用ab18217于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt | Use 1µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Entrez Gene: 991 Human
Omim: 603618 Human
SwissProt: Q12834 Human
Unigene: 524947 Human
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Flow Cytometry - Anti-Cdc20 antibody [BA8] (ab18217)
Overlay histogram showing LoVo cells stained with ab18217 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18217, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.