Anti-RanBP1抗体
参阅全部 RanBP1 一抗
山羊多克隆抗体to RanBP1
Goat
适用于: Flow Cyt, ICC/IF, WB, IHC-Pmore details
与反应: Human
Synthetic peptide corresponding to Human RanBP1 aa 150 to the C-terminus.
Database link: P43487
WB: Human duodenum lysate. A431, HeLa and MCF7 whole cell lysate. ICC/IF: HEK293 and HeLa cells. Flow Cyt: HEK293 cells
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
浓度
100 µg 浓度为 0.5 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab17034于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt | Use a concentration of 10 µg/ml. | |
| ICC/IF | Use a concentration of 10 µg/ml. | |
| WB | Use a concentration of 0.05 - 0.1 µg/ml. Detects a band of approximately 28-30 kDa (predicted molecular weight: 23 kDa). A 1 hour primary incubation is recommended for this product. | |
| IHC-P | Use a concentration of 3 - 5 µg/ml. |
Entrez Gene: 5902 Human
Omim: 601180 Human
SwissProt: P43487 Human
Unigene: 24763 Human
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Flow Cytometry - Anti-RanBP1 antibody (ab17034)
Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Western blot - Anti-RanBP1 antibody (ab17034)
Anti-RanBP1 antibody (ab17034) at 0.3 µg/ml + Human duodenum lysate (RIPA buffer) at 35 µg
Predicted band size: 23 kDa
Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence - Anti-RanBP1 antibody (ab17034)
Immunofluorescence analysis of paraformaldehyde fixed HEK-293 (Human epithelial cell line from embryonic kidney) cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/ml) followed by Alexa Fluor 488® secondary antibody (2 µg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue).
Negative control: Non-immunized goat IgG (10 µg/ml) followed by Alexa Fluor 488® secondary antibody (2 µg/ml).
Western blot - Anti-RanBP1 antibody (ab17034)
Lane 1 : Anti-RanBP1 antibody (ab17034) at 0.03 µg/ml
Lane 2 : Anti-RanBP1 antibody (ab17034) at 0.5 µg/ml
Lane 3 : Anti-RanBP1 antibody (ab17034) at 0.1 µg/ml
Lane 1 : A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 23 kDa
Detected by chemiluminescence.
Lysates in RIPA buffer.
Immunocytochemistry/ Immunofluorescence - Anti-RanBP1 antibody (ab17034)
Immunofluorescence analysis of paraformaldehyde fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/ml) followed by Alexa Fluor 488® secondary antibody (2 µg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue).
Negative control: Non-immunized goat IgG (10 µg/ml) followed by Alexa Fluor 488® secondary antibody (2 µg/ml).
