Anti-ABCA1 antibody [AB.H10]

• 产品名称

  Anti-ABCA1抗体[AB.H10]
  参阅全部 ABCA1 一抗

• 描述

  小鼠单克隆抗体[AB.H10] to ABCA1

• 宿主

  Mouse

• 经测试应用

  适用于: IHC-P, Flow Cyt, WBmore details

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Recombinant fragment corresponding to Human ABCA1 aa 1800-2260.
  Database link: O95477

• 阳性对照

• testis, liver, and brain tissue (negative control: muscle tissue)

• 常规说明

  This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.20
  Preservative: 0.05% Sodium azide
  Constituent: PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 纯化说明

  Protein A purified from TCS

• 克隆

  单克隆

• 克隆编号

  AB.H10

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab18180于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 19 Human

• Entrez Gene: 11303 Mouse

• Omim: 600046 Human

• SwissProt: O95477 Human

• SwissProt: P41233 Mouse

• Unigene: 429294 Human

• Unigene: 277376 Mouse

• 别名

• ABC1 antibody

• ABCA 1 antibody

• ABCA1 antibody

• ABCA1_HUMAN antibody

• ATP binding Cassette 1 antibody

• ATP binding cassette sub family A ABC1 member 1 antibody

• ATP binding cassette sub family A member 1 antibody

• ATP binding Cassette Transporter 1 antibody

• ATP-binding cassette 1 antibody

• ATP-binding cassette sub-family A member 1 antibody

• ATP-binding cassette transporter 1 antibody

• CERP antibody

• Cholesterol efflux regulatory protein antibody

• FLJ14958 antibody

• HDLDT1 antibody

• Membrane bound antibody

• MGC164864 antibody

• MGC165011 antibody

• TD antibody

• ABC 1 antibody

• ABC Transporter 1 antibody

• ABC-1 antibody

• TGD antibody

• 

  Flow Cytometry - Anti-ABCA1 antibody [AB.H10] (ab18180)

  Overlay histogram showing HepG2 cells stained with ab18180 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18180, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
  
  

• 

  Western blot - Anti-ABCA1 antibody [AB.H10] (ab18180)

  Western blot analysis of ABCA1 induction by cholesterol (+) in human fibroblast cells from different patients (1,2,3,4). Simultaneous bloting with anti-actin antibody was used for protein loading control. Western blot analysis of ABCA1 induction by cholesterol (+) in human fibroblast cells from different patients (1,2,3,4). Simultaneous blotting with anti-actin antibody was used for protein loading control.

• 

  Western blot - Anti-ABCA1 antibody [AB.H10] (ab18180)

  All lanes : Anti-ABCA1 antibody [AB.H10] (ab18180) at 1/1000 dilution
  
  Lanes 1 & 3 & 5 : Wild type mouse liver tissue lysate
  Lanes 2 & 4 & 6 : Knockout mouse liver tissue lysate
  
  Lysates/proteins at 100 µg per lane.
  
  Predicted band size: 254 kDa
  Observed band size: 254 kDa
  
  
  
  

  The lower bands are from the secondary anti-mouse antibody reacting to the endogenous mouse antibodies from the tissue.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody [AB.H10] (ab18180)

  Immunoreactivity of pancreas tissue for ABCA1 protein. Left panel: strong immunopositivity of exocrine glandular cells of the pancreas, especially in the basal region (arrows). Interstitial microvascular cells are apparently negative. Cells within the islet were weakly positive (data not shown). Right panel: the negative control. Hematoxylin counterstain.