Anti-Myc tag antibody [Myc.A7]

• 产品名称

  Anti-Myc tag抗体[Myc.A7]
  参阅全部 Myc tag 一抗

• 描述

  小鼠单克隆抗体[Myc.A7] to Myc tag

• 宿主

  Mouse

• 特异性

  Recognizes overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells.

• 经测试应用

  适用于: ELISA, Dot blot, WB, IPmore details

• 免疫原

  Synthetic peptide:

  EQKLISEEDL

  , corresponding to amino acids 410-419 of Human Myc.

  Run BLAST with Run BLAST with 

• 阳性对照

• WB: HEK-293 whole cell lysate ICC/IF: rat primary hippocampal neurons

• 常规说明

  This product was changed from ascites to tissue culture supernatant on 25th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.05% Sodium azide
  Constituent: PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 纯化说明

  Protein A affinity chromatography

• 克隆

  单克隆

• 克隆编号

  Myc.A7

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab18185于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 别名

• c-myc tag antibody

• Myc Epitope Tag antibody

• 

  Western blot - Anti-Myc tag antibody [Myc.A7] (ab18185)

  All lanes : Anti-Myc tag antibody [Myc.A7] (ab18185) at 1/1000 dilution
  
  Lane 1 : Wild-type HEK-293T cell lysate
  Lane 2 : MYC CRISPR-Cas9 edited HEK-293T cell lysate
  Lane 3 : Jurkat cell lysate
  Lane 4 : SH-SY5Y cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Performed under reducing conditions.
  
  
  

  False colour image of Western blot: Anti-Myc tag antibody [Myc.A7] staining at 1/1000 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab18185 was shown to bind specifically to Myc tag. A band was observed at 57 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in MYC CRISPR-Cas9 edited cell line ab256500 (CRISPR-Cas9 edited cell lysate ab263850). The band observed in the CRISPR-Cas9 edited lysate lane below 57 kDa is likely to represent a truncated form of Myc tag. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and MYC CRISPR-Cas9 edited HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.

• 

  Western blot - Anti-Myc tag antibody [Myc.A7] (ab18185)

  All lanes : Anti-Myc tag antibody [Myc.A7] (ab18185) at 1/1000 dilution
  
  Lane 1 : HEK293 lysate - untransfected
  Lane 2 : HEK293 lysate - transfected with Myc-tagged protein