Anti-Hsp27 (phospho S86) antibody

• 产品名称

  Anti-Hsp27 (phospho S86)抗体
  参阅全部 Hsp27 一抗

• 描述

  兔多克隆抗体to Hsp27 (phospho S86)

• 宿主

  Rabbit

• 特异性

  Lysates prepared from NIH3T3 cells were immunoblotted in the presence of non-phosphopeptide corresponding to the immunogen, a generic phosphoserine-containing peptide or the phosphopeptide immunogen. The data show that only the peptide corresponding to the mouse phospho S86 HSP27 blocks the antibody signal, thereby demonstrating the specificity of the antibody. The signal was completely removed by lambda phosphatase treatment demonstrating that the antibody interacts specifically with the phosphorylated protein.

• 经测试应用

  适用于: WB, ICCmore details

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Synthetic peptide corresponding to Mouse Hsp27 (phospho S86).

• 阳性对照

• WB: NIH/3T3 cells. ICC: HeLa cells.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.30
  Preservative: 0.05% Sodium azide
  Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

• 浓度

• 50 µl 浓度为 0.25 mg/ml

• 纯度

  Immunogen affinity purified

• 纯化说明

  The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated HSP25 (the mouse homolog of human HSP27). The final product is generated by affinity chromatography using an HSP25-derived peptide that is phosphorylated at serine 86.

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab17938于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 3315 Human

• Entrez Gene: 15507 Mouse

• Omim: 602195 Human

• SwissProt: P04792 Human

• SwissProt: P14602 Mouse

• Unigene: 520973 Human

• Unigene: 13849 Mouse

• 别名

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• Estrogen regulated 24 kDa protein antibody

• Estrogen-regulated 24 kDa protein antibody

• Heat shock 25kDa protein 1 antibody

• Heat shock 27 kDa protein antibody

• Heat shock 27kD protein 1 antibody

• Heat shock 27kDa protein 1 antibody

• Heat shock 28kDa protein 1 antibody

• Heat Shock Protein 27 antibody

• Heat shock protein beta 1 antibody

• Heat shock protein beta-1 antibody

• heat shock protein family B (small) member 1 antibody

• HEL-S-102 antibody

• HMN2B antibody

• HS.76067 antibody

• Hsp 25 antibody

• HSP 27 antibody

• Hsp 28 antibody

• Hsp B1 antibody

• Hsp25 antibody

• HSP27 antibody

• Hsp28 antibody

• HspB1 antibody

• HSPB1_HUMAN antibody

• SRP27 antibody

• Stress responsive protein 27 antibody

• Stress-responsive protein 27 antibody

• Heat shock 27kDa protein antibody

• 28 kDa heat shock protein antibody

• CMT2F antibody

• 

  Western blot - Anti-Hsp27 (phospho S86) antibody (ab17938)

  Hsp25 (phospho S86) antibody image 6534.

  Western blot using ab17938 on NIH3T3 cells treated with anisomycin.
  Lane 1: unstimulated cells
  Lane 2: cells stimulated with anisomycin
  Lane 3: cells stimulated with anisomycin. Antibody blocked with the non-phosphopeptide corresponding to the immunogen
  Lane 4: cells stimulated with anisomycin. Antibody blocked with generic phosphoserine-containing peptide
  Lane 5: cells stimulated with anisomycin. Antibody blocked with the phosphopeptide immunogen
  Lane 6: cells stimulated with anisomycin and treated with lambda phosphatase
  10-30 µg of cell lysate can be loaded when using similar lysates with this antibody. Samples were run using SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature, then incubated with ab17938 for one hour at room temperature in 3% BSA-TBST buffer, following prior incubation with blocking

• 

  Immunocytochemistry - Anti-Hsp27 (phospho S86) antibody (ab17938)

  HeLa cells stained for Hsp27 (green) using ab17938 at 1/250 dilution in ICC/IF. It was followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin at 1/300 dilution. Panel d is a merged image showing cytoplasmic localization. Panel e is untreated cell with no signal. Panel f is a no primary antibody control.